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Cell No. : Cell Name
RCB3010 : RPMI8226  update : 2020/09/24
CommentHuman cell line derived from myeloma. IgG producer. TKG0243 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Benda et al.
Year of deposit 2010
Original cell TKG0243
Animal human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Case history myeloma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0243)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution, pipetting
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
deposit info
lot info
Reference information Reference 2
User's Publication 3

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5498  Moore GE, Kitamura H.  Cell line derived from patient with myeloma.  N Y State J Med  1968  68(15):2054-60  PubMed ID: 5243066
5497  Matsuoka Y, Moore GE, Yagi Y, Pressman D.  Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma.  Proc Soc Exp Biol Med  1967  125(4):1246-50  PubMed ID: 6042436

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User's Publication
12010  Teratake Y, Takashina T, Iijima K, Sakuma T, Yamamoto T, Ishizaka Y.  Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells.  Br. J. Cancer  2020    PubMed ID: 31959920
4270  Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki Y, Saito M, Iwamura H, Kanakura Y.  Molecular targeting of inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via ROS/MAPK pathway.  Leuk. Lymphoma  2018    PubMed ID: 28730859
4500  Tanaka M, Zhu Y, Shionyu M, Ota N, Shibata N, Watanabe C, Mizusawa A, Sasaki R, Mizukami T, Shiina I, Hasegawa M.  Ridaifen-F conjugated with cell-penetrating peptides inhibits intracellular proteasome activities and induces drug-resistant cell death.  Eur J Med Chem  2018  146:636-650  PubMed ID: 29407987

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