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Cell No. : Cell Name
RCB3010 : RPMI8226  update : 2023/10/16
CommentHuman cell line derived from myeloma. IgG producer. TKG0243 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Benda et al.
Year of deposit 2010
Original cell TKG0243
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Disease name myeloma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0243)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0014
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution, pipetting
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 8


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Reference
5498  Moore GE, Kitamura H.  Cell line derived from patient with myeloma.  N Y State J Med  1968  68(15):2054-60  PubMed ID: 5243066  
5497  Matsuoka Y, Moore GE, Yagi Y, Pressman D.  Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma.  Proc Soc Exp Biol Med  1967  125(4):1246-50  PubMed ID: 6042436   DOI: 10.3181/00379727-125-32327

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User's Publication
19629  Acceleration of Protein Degradation by 20S Proteasome-Binding Peptides Generated by In Vitro Artificial Evolution  Acceleration of Protein Degradation by 20S Proteasome-Binding Peptides Generated by In Vitro Artificial Evolution  Int J Mol Sci  2023  24(24):17486  PubMed ID: 38139315   DOI: 10.3390/ijms242417486
12010  Teratake Y, Takashina T, Iijima K, Sakuma T, Yamamoto T, Ishizaka Y.  Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells.  Br. J. Cancer  2020    PubMed ID: 31959920   DOI: 10.1038/s41416-019-0708-y
4270  Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki Y, Saito M, Iwamura H, Kanakura Y.  Molecular targeting of inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via ROS/MAPK pathway.  Leuk. Lymphoma  2018    PubMed ID: 28730859   DOI: 10.1080/10428194.2017.1339878
4500  Tanaka M, Zhu Y, Shionyu M, Ota N, Shibata N, Watanabe C, Mizusawa A, Sasaki R, Mizukami T, Shiina I, Hasegawa M.  Ridaifen-F conjugated with cell-penetrating peptides inhibits intracellular proteasome activities and induces drug-resistant cell death.  Eur J Med Chem  2018  146:636-650  PubMed ID: 29407987   DOI: 10.1016/j.ejmech.2018.01.045
15045  Tsubaki M, Takeda T, Tomonari Y, Mashimo K, Koumoto YI, Hoshida S, Itoh T, Imano M, Satou T, Sakaguchi K, Nishida S.  The MIP-1α autocrine loop contributes to decreased sensitivity to anticancer drugs  J Cell Physiol  2018  233(5):4258-4271.  PubMed ID: 29057477   DOI: 10.1002/jcp.26245
14622  Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y.  Cytokine-mediated activation of human ex vivo-expanded Vγ9Vδ2 T cells  Oncotarget  2017  8(28):45928-45942  PubMed ID: 28521284   DOI: 10.18632/oncotarget.17498
12516  Ono M, Takimoto R, Osuga T, Okagawa Y, Hirakawa M, Yoshida M, Arihara Y, Uemura N, Hayasaka N, Miura S, Matsuno T, Tamura F, Sato Y, Sato T, Iyama S, Miyanishi K, Takada K, Kobune M, Kato J.  Targeting Notch-1 positive acute leukemia cells by novel fucose-bound liposomes carrying daunorubicin  Oncotarget  2016  7(25):38586-38597  PubMed ID: 27233074   DOI: 10.18632/oncotarget.9558
14309  Ninomiya S, Kawano M, Abe T, Ishikawa T, Takahashi M, Tamura M, Takahashi Y, Nashimoto M.  Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines  PLoS One  2015  10(3):e0118631  PubMed ID: 25730316   DOI: 10.1371/journal.pone.0118631



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