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Cell No. : Cell Name
RCB3007 : MOPC-70A  update : 2024/01/17
CommentMouse cell line derived from myeloma. This cell line was established from a cell line maintained by in vivo transplantation. TKG0167 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2010
Original cell TKG0167
Animal _mouse < Mammals
Strain name BALB/c
Genus Mus
Species musculus
Gender Female
Tissue abdominal cavity
Disease name myeloma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0167)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_T766
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
5494  Tung JS, Scheid MP, Pierotti MA, Hämmerling U, Boyse EA.  Structural features and selective expression of three Ly-5+ cell-surface molecules.  Immunogenetics  1981  14(1-2):101-6  PubMed ID: 7035346   DOI: 10.1007/bf00344303
5495  Kaneko Y, Natsuume-Sakai S, Moriwaki K, Migita S.  F1 hybrid resistance to murine plasmacytoma MOPC-70A: hybrid resistance to MOPC-70A controlled by a locus linked to H-2.  J Immunol  1980  125(5):2031-8  PubMed ID: 7430619  
5496  Richards JM, Jacobson JB, Stackpole CW.  Antigenic modulation in vitro. III. Failure to modulate H-2 antigens on several mouse tumors.  J Natl Cancer Inst  1979  62(4):825-32  PubMed ID: 311870  
5493  Aoki T, Takahashi T.  Viral and cellular surface antigens of murine leukemias and myelomas. Serological analysis by immunoelectron microscopy.  J Exp Med  1972  135(3):443-57  PubMed ID: 5058232   DOI: 10.1084/jem.135.3.443

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