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Cell No. : Cell Name
RCB2992 : HCT116-DNAPKcs(+/-)  update : 2022/05/30
CommentSubline of HCT116 cell line lacking DNAPKcs gene in one allele. Loss of the DNA mismatch repair gene (hMLH1).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shiomi, Tadahiro
Originator Shiomi, Naoko & Mori Masahiko
Year of deposit 2009
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue colon
Classification cancer
Recombinant recombinant
Exogene Neomycin resistance gene
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1R01
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343

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