Cell No. : Cell Name RCB2981 : HCT116-XRCC4(-/-)  update : 2023/10/16
Comment			Subline of HCT116 cell line lacking XRCC4 gene in both alleles.　Loss of the DNA mismatch repair gene (hMLH1).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Shiomi, Tadahiro
		Originator	Shiomi, Naoko & Mori Masahiko
		Year of deposit	2009
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Tissue	colon
		Classification	cancer
		Recombinant	recombinant
		Exogene	Puromycin resistance gene, Neomycin resistance gene
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_T773
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	2

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Reference

18268  Katsube T, Mori M, Tsuji H, Shiomi T, Shiomi N, Onoda M.  Differences in sensitivity to DNA-damaging Agents between XRCC4- and Artemis-deficient human cells  J Radiat Res  2011  52(4):415-24  PubMed ID: 21785230   DOI: 10.1269/jrr.10168

5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343

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User's Publication

16354  Koike M, Yutoku Y, Koike A.  Feline XRCC4 undergoes rapid Ku-dependent recruitment to DNA damage sites  FEBS Open Bio  2022  Jan 9  PubMed ID: 35000298   DOI: 10.1002/2211-5463.13363

15210  Guo C, Nakazawa Y, Woodbine L, Björkman A, Shimada M, Fawcett H, Jia N, Ohyama K, Li TS, Nagayama Y, Mitsutake N, Pan-Hammarström Q, Gennery AR, Lehmann AR, Jeggo PA, Ogi T.  XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency  J Allergy Clin Immunol  2015  136(4):1007-17  PubMed ID: 26255102   DOI: 10.1016/j.jaci.2015.06.007