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Cell No. : Cell Name
RCB2981 : HCT116-XRCC4(-/-)  update : 2024/06/10
CommentSubline of HCT116 cell line lacking XRCC4 gene in both alleles. Loss of the DNA mismatch repair gene (hMLH1).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shiomi, Tadahiro
Originator Shiomi, Naoko & Mori Masahiko
Year of deposit 2009
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue colon
Classification cancer
Recombinant recombinant
Exogene Puromycin resistance gene, Neomycin resistance gene
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_T773
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
18268  Katsube T, Mori M, Tsuji H, Shiomi T, Shiomi N, Onoda M.  Differences in sensitivity to DNA-damaging Agents between XRCC4- and Artemis-deficient human cells  J Radiat Res  2011  52(4):415-24  PubMed ID: 21785230   DOI: 10.1269/jrr.10168
5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343  

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User's Publication
16354  Koike M, Yutoku Y, Koike A.  Feline XRCC4 undergoes rapid Ku-dependent recruitment to DNA damage sites  FEBS Open Bio  2022  Jan 9  PubMed ID: 35000298   DOI: 10.1002/2211-5463.13363
15210  Guo C, Nakazawa Y, Woodbine L, Björkman A, Shimada M, Fawcett H, Jia N, Ohyama K, Li TS, Nagayama Y, Mitsutake N, Pan-Hammarström Q, Gennery AR, Lehmann AR, Jeggo PA, Ogi T.  XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency  J Allergy Clin Immunol  2015  136(4):1007-17  PubMed ID: 26255102   DOI: 10.1016/j.jaci.2015.06.007



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