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Cell No. : Cell Name
RCB2979 : HCT116  update : 2021/01/08
CommentHuman cell line derived from colon cancer. Loss of the DNA mismatch repair gene (hMLH1).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shiomi, Tadahiro
Year of deposit 2009
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue colon
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 61


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Reference
5492  Long BH, Willson JK, Brattain DE, Musial S, Brattain MG.  Effects of mitomycin on human colon carcinoma cells.  J Natl Cancer Inst  1984  73(4):787-92  PubMed ID: 6434780
5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343

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User's Publication
13604  Jiang W, Jia N, Guo C, Wen J, Wu L, Ogi T, Zhang H.  Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder  Biochim Biophys Acta Mol Basis Dis  2021  1867(6):166106  PubMed ID: 33631320
20083  Hama K, Fujiwara Y, Hayama T, Ozawa T, Nozawa K, Matsuda K, Hashiguchi Y, Yokoyama K.  Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues.  Sci Rep  2021  11(1):6163  PubMed ID: 33731797
13454  Wataru Matsunaga, Misa Ichikawa, Takahiro Ishikawa, Akinobu Gotoh  Lentiviral vector-mediated transfection of human cancer cell lines with tumor suppressor genes inhibits proliferation in vitro  Personalized Medicine Universe  2020/1/1   
6918  Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M, Osada H.  Identification of a Small Compound Targeting PKM2-Regulated Signaling Using 2D Gel Electrophoresis-Based Proteome-wide CETSA.  Cell Chem Biol  2020  27(2):186-196  PubMed ID: 31813846
9166  Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel, Chaowan Guo, Yasukazu Daigaku, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato Mitsutake, Takayoshi Suganami, Akio Masuda, Kinji Ohno, Shinichiro Nakada, Tomoji Mashimo, Koji Yamanaka, Martijn S Luijsterburg, Tomoo Ogi   Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair  Cell  2020  180 (6), 1228-1244  PubMed ID: 32142649
9913  Satoshi Inoue, Takumi Tsunoda, Miho Riku, Hideaki Ito, Akihito Inoko, Hideki Murakami, Masahide Ebi, Naotaka Ogasawara, Ira Pastan, Kunio Kasugai, Kenji Kasai, Hiroshi Ikeda, Shingo Inaguma  Diffuse Mesothelin Expression Leads to Worse Prognosis Through Enhanced Cellular Proliferation in Colorectal Cancer  Oncol Lett  2020  19(3):1741-1750  PubMed ID: 32194667
12035  Kitao A, Kawamoto S, Kurata K, Hayakawa I, Yamasaki T, Matsuoka H, Sumi Y, Kakeji Y, Kamesaki T, Minami H.  Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.  Int. J. Hematol.  2020    PubMed ID: 31997080
12130  Sanada S, Suzuki T, Nagata A, Hashidume T, Yoshikawa Y, Miyoshi N.  Intestinal microbial metabolite stercobilin involvement in the chronic inflammation of ob/ob mice.  Sci Rep  2020    PubMed ID: 32296105
12136  Choodej, Khanitha Pudhom  Cycloartane triterpenoids from the leaves of Euphorbia neriifolia  Phytochemistry Letters  2020   
12150  Amira Mira, Mohamed A. Sabry, Kuniyoshi Shimizu & Fatma M. Abdel Bar  A new pimarane-type diterpene obtained by biotransformation inhibits human HCT-116 colorectal carcinoma through inhibition of LTA4H activity  Original Research  2020   
13059  Ratsada P, Hijiya N, Hidano S, Tsukamoto Y, Nakada C, Uchida T, Kobayashi T, Moriyama M.  DUSP4 is involved in the enhanced proliferation and survival of DUSP4-overexpressing cancer cells.  Biochem Biophys Res Commun  2020    PubMed ID: 32505357
13210  Wada Y, Morine Y, Imura S, Ikemoto T, Saito Y, Takasu C, Yamada S, Shimada M.  HIF-1α expression in liver metastasis but not primary colorectal cancer is associated with prognosis of patients with colorectal liver metastasis.  World J Surg Oncol  2020    PubMed ID: 32895059
13220  Miyano K, Okamoto S, Yamauchi A, Kajikawa M, Kiyohara T, Taura M, Kawai C, Kuribayashi F.  Constitutive activity of NADPH oxidase 1 (Nox1) that promotes its own activity suppresses the colon epithelial cell migration.  Free Radic Res  2020    PubMed ID: 32924676
13390  Murakami H, Tamura N, Enomoto Y, Shimasaki K, Kurosawa K, Hanada K.  Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.  PLoS One  2020    PubMed ID: 33347465
13502  Nambara S, Masuda T, Kobayashi Y, Sato K, Tobo T, Koike K, Noda M, Ogawa Y, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Mimori K.  GTF2IRD1 on chromosome 7 is a novel oncogene regulating the tumor-suppressor gene TGFβR2 in colorectal cancer  Cancer Sci  2020  111(2):343-355  PubMed ID: 31758608
13505  Oka S, Kuniba R, Tsuboi N, Tsuchida S, Ushida K, Tomoshige S, Kuramochi K.  Isolation, synthesis, and biological activities of a bibenzyl from Empetrum nigrum var. japonicum  Biosci Biotechnol Biochem  2020  84(1):31-36  PubMed ID: 31794330
11388  Kitada K, Pu F, Toi M.  Occurrence of senescence-escaping cells in doxorubicin-induced senescence is enhanced by PD0332991, a cyclin-dependent kinase 4/6 inhibitor, in colon cancer HCT116 cells.  Oncol Lett  2019    PubMed ID: 30655877
11359  Shimizu D, Masuda T, Sato K, Tsuruda Y, Otsu H, Kuroda Y, Eguchi H, Kodera Y, Mimori K.  CRMP5-associated GTPase (CRAG) Is a Candidate Driver Gene for Colorectal Cancer Carcinogenesis.  Anticancer Res.  2019    PubMed ID: 30591445
11408  Suzuki N, Niikura R, Ihara S, Hikiba Y, Kinoshita H, Higashishima N, Hayakawa Y, Yamada A, Hirata Y, Nakata R, Okamoto M, Sano M, Kushiyama A, Ichinose M, Woods SL, Worthley D, Iwamoto Y, Koike K.  Alpha-Blockers As Colorectal Cancer Chemopreventive: Findings from a Case-Control Study, Human Cell Cultures, and In Vivo Preclinical Testing.  Cancer Prev Res (Phila)  2019    PubMed ID: 30700439
11517  Sekido M, Fujita KI, Kubota Y, Ishida H, Takahashi T, Ohkuma R, Tsunoda T, Ishikawa F, Shibanuma M, Sasaki Y.  Rabeprazole intake does not affect systemic exposure to capecitabine and its metabolites, 5'-deoxy-5-fluorocytidine, 5'-deoxy-5-fluorouridine, and 5-fluorouracil.  Cancer Chemother. Pharmacol.  2019    PubMed ID: 30972456
11725  Kawasaki Y, Miyamoto M, Oda T, Matsumura K, Negishi L, Nakato R, Suda S, Yokota N, Shirahige K, Akiyama T.  The novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis.  EMBO Rep.  2019    PubMed ID: 31353791
11835  Yokoyama Y, Sakatani T, Wada R, Ishino K, Kudo M, Koizumi M, Yamada T, Yoshida H, Naito Z.  In vitro and in vivo studies on the association of long non‑coding RNAs H19 and urothelial cancer associated 1 with the susceptibility to 5‑fluorouracil in rectal cancer.  Int. J. Oncol.  2019    PubMed ID: 31638183
11851  Fujitani N, Yoneda A, Takahashi M, Takasawa A, Aoyama T, Miyazaki T.  Silencing of Glutathione S-Transferase Pi Inhibits Cancer Cell Growth via Oxidative Stress Induced by Mitochondria Dysfunction.  Sci Rep  2019    PubMed ID: 31611630
11864  Matsumoto Y, Kuriki H, Kitamura T, Takahashi D, Toshima K.  Total Synthesis and Structure-Activity Relationship Study of Vineomycin A1.  J. Org. Chem.  2019    PubMed ID: 31642324
11905  Igarashi H, Taniguchi H, Nosho K, Ishigami K, Koide H, Mitsuhashi K, Okita K, Takemasa I, Imai K, Nakase H.  PRDM14 promotes malignant phenotype and correlates with poor prognosis in colorectal cancer.  Clin Transl Oncol  2019    PubMed ID: 31741141
11910  Ujiie D, Okayama H, Saito K, Ashizawa M, Thar Min AK, Endo E, Kase K, Yamada L, Kikuchi T, Hanayama H, Fujita S, Sakamoto W, Endo H, Saito M, Mimura K, Saze Z, Momma T, Ohki S, Kono K.  KRT17 as a prognostic biomarker for stage II colorectal cancer.  Carcinogenesis  2019    PubMed ID: 31754689
12405  Kouyama Y, Masuda T, Fujii A, Ogawa Y, Sato K, Tobo T, Wakiyama H, Yoshikawa Y, Noda M, Tsuruda Y, Kuroda Y, Eguchi H, Ishida F, Kudo SE, Mimori K.  Oncogenic splicing abnormalities induced by DEAD-Box Helicase 56 amplification in colorectal cancer  Cancer Sci   2019  110(10):3132-3144  PubMed ID: 31390121
12420  Aoki T, Nishita M, Sonoda J, Ikeda T, Kakeji Y, Minami Y.  Intraflagellar transport 20 promotes collective cancer cell invasion by regulating polarized organization of Golgi-associated microtubules  Cancer Sci  2019  110(4):1306-1316  PubMed ID: 30742741
12421  Koike M, Yutoku Y, Koike A.  Feline XLF accumulates at DNA damage sites in a Ku-dependent manner  FEBS Open Bio  2019  9(6):1052-1062  PubMed ID: 31115163
12422  Miyagi A, Kawashiri T, Shimizu S, Shigematsu N, Kobayashi D, Shimazoe T.  Dimethyl Fumarate Attenuates Oxaliplatin-Induced Peripheral Neuropathy without Affecting the Anti-tumor Activity of Oxaliplatin in Rodents  Biol Pharm Bull  2019  42(4):638-644.  PubMed ID: 30930422
12423  Wang-Bishop L, Chen Z, Gomaa A, Lockhart AC, Salaria S, Wang J, Lewis KB, Ecsedy J, Washington K, Beauchamp RD, El-Rifai W.  Inhibition of AURKA Reduces Proliferation and Survival of Gastrointestinal Cancer Cells With Activated KRAS by Preventing Activation of RPS6KB1  Gastroenterology  2019  156(3):662-675.e7  PubMed ID: 30342037
12424  Kawashiri T, Shimizu S, Shigematsu N, Kobayashi D, Shimazoe T.  Donepezil ameliorates oxaliplatin-induced peripheral neuropathy via a neuroprotective effect  J Pharmacol Sci  2019  140(3):291-294  PubMed ID: 31377017
12517  Satria D, Amen Y, Niwa Y, Ashour A, Allam AE, Shimizu K.  Lucidumol D, a new lanostane-type triterpene from fruiting bodies of Reishi (Ganoderma lingzhi)  Nat Prod Res  2019  33(2):189-195  PubMed ID: 29457741
9501  Shiota M, Naya M, Yamamoto T, Hishiki T, Tani T, Takahashi H, Kubo A, Koike D, Itoh M, Ohmura M, Kabe Y, Sugiura Y, Hiraoka N, Morikawa T, Takubo K, Suina K, Nagashima H, Sampetrean O, Nagano O, Saya H, Yamazoe S, Watanabe H, Suematsu M.  Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.  Nat Commun  2018  9:1561  PubMed ID: 29674746
10580  Mito A, Nakano Y, Saitoh T, Gouraud SSS, Yamaguchi Y, Sato T, Sasaki N, Kojima-Aikawa K.  Lectin ZG16p inhibits proliferation of human colorectal cancer cells via its carbohydrate-binding sites.  Glycobiology  2018  28:21-31  PubMed ID: 29069492
10606  Kanno E, Kawasaki O, Takahashi K, Takano K, Endo T.  DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, is a putative tumor suppressor.  Exp. Cell Res.  2018  362:111-120  PubMed ID: 29129563
10702  Horibe S, Tanahashi T, Kawauchi S, Murakami Y, Rikitake Y.  Mechanism of recipient cell-dependent differences in exosome uptake.  BMC Cancer  2018  18:47  PubMed ID: 29306323
10751  Noguchi A, Adachi S, Yokota N, Hatta T, Natsume T, Kawahara H.  ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest.  Biol Open  2018  7:  PubMed ID: 29449217
10757  Seiler M, Yoshimi A, Darman R, Chan B, Keaney G, Thomas M, Agrawal AA, Caleb B, Csibi A, Sean E, Fekkes P, Karr C, Klimek V, Lai G, Lee L, Kumar P, Lee SC, Liu X, Mackenzie C, Meeske C, Mizui Y, Padron E, Park E, Pazolli E, Peng S, Prajapati S, Taylor J, Teng T, Wang J, Warmuth M, Yao H, Yu L, Zhu P, Abdel-Wahab O, Smith PG, Buonamici S.  H3B-8800, an orally available small-molecule splicing modulator, induces lethality in spliceosome-mutant cancers.  Nat. Med.  2018  24:497-504  PubMed ID: 29457796
10847  Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S, Tanaka T, Yokoi K, Nishizawa N, Yokota K, Kosaka Y, Sato T, Watanabe M, Yamashita K.  Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer.  PLoS ONE  2018  13:e0194785  PubMed ID: 29746493
11009    Evidence suggesting sphingosine 1-phosphate may be actively generated, degraded and transported to extracellular spaces with increased S1P2 and S1P3 expressions in colon cancer  Clinical Colorectal Cancer  2018  17, e171-e182  PubMed ID: 29223361
11087  Thar Min AK, Okayama H, Saito M, Ashizawa M, Aoto K, Nakajima T, Saito K, Hayase S, Sakamoto W, Tada T, Hanayama H, Saze Z, Momma T, Ohki S, Sato Y, Motoyama S, Mimura K, Kono K.  Epithelial-mesenchymal transition-converted tumor cells can induce T-cell apoptosis through upregulation of programmed death ligand 1 expression in esophageal squamous cell carcinoma.  Cancer Med  2018    PubMed ID: 29855157
4422  Enos Tangke Arung, Rudianto Amirta, Qinchang Zhu, Yhiya Amen, Kuniyoshi Shimizu  Effect of wood, bark and leaf extracts of Macaranga trees on cytotoxic activity in some cancer and normal cell lines  Journal of the Indian Academy of Wood Science  2018  5, 115–119 
4195  Ishigaki Y, Sato K.  Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.  Micromachines (Basel)  2018    PubMed ID: 30424205
4196  Wada F, Koga H, Akiba J, Niizeki T, Iwamoto H, Ikezono Y, Nakamura T, Abe M, Masuda A, Sakaue T, Tanaka T, Kakuma T, Yano H, Torimura T.  High expression of CD44v9 and xCT in chemoresistant hepatocellular carcinoma: Potential targets by sulfasalazine.  Cancer Sci.  2018    PubMed ID: 29981246
4344  Li HK, Hasegawa S, Nakajima NI, Morokoshi Y, Minegishi K, Nagatsu K.  Targeted cancer cell ablation in mice by an α-particle-emitting astatine-211-labeled antibody against major histocompatibility complex class I chain-related protein A and B.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 30409432
9847  Koike M, Yutoku Y, Koike A.  Cloning, localization and focus formation at DNA damage sites of canine XRCC4.  J. Vet. Med. Sci.  2017  78:1865-1871  PubMed ID: 27644316
10185  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10296  Gombodorj N, Yokobori T, Yoshiyama S, Kawabata-Iwakawa R, Rokudai S, Horikoshi I, Nishiyama M, Nakano T.  Inhibition of Ubiquitin-conjugating Enzyme E2 May Activate the Degradation of Hypoxia-inducible Factors and, thus, Overcome Cellular Resistance to Radiation in Colorectal Cancer.  Anticancer Res.  2017  37:2425-2436  PubMed ID: 28476810
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
10520  Koike M, Yutoku Y, Koike A.  Cloning of canine Ku80 and its localization and accumulation at DNA damage sites.  FEBS Open Bio  2017  7:1854-1863  PubMed ID: 29226073
10535  Ohashi W, Yamamine N, Imura J, Hattori Y.  SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex.  Biochem. Biophys. Res. Commun.  2017  493:1342-1348  PubMed ID: 28970068
4194  Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Ishida S, Kikuchi K, Kakiki M, Kanamori T.  A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform.  Lab Chip  2017    PubMed ID: 29184959
10028  Michinori Takashina, Sayaka Inoue, Kei Tomihara, Kengo Tomita, Kohshi Hattori, Qing-Li Zhao, Tokiko Suzuki, Makoto Noguchi, Wakana Ohashi, Yuichi Hattori  Different Effect of Resveratrol to Induction of Apoptosis Depending on the Type of Human Cancer Cells  Int J Oncol  2017  50(3):787-797.  PubMed ID: 28197625
9637  Li W, Itou J, Tanaka S, Nishimura T, Sato F, Toi M.  A homeobox protein, NKX6.1, up-regulates interleukin-6 expression for cell growth in basal-like breast cancer cells.  Exp. Cell Res.  2016  343:177-189  PubMed ID: 27032575
10913    Clinical potential of DAC-70 sol  Integr Mol Med  2016  3: 645-648 
12896  Koizumi S, Irie T, Hirayama S, Sakurai Y, Yashiroda H, Naguro I, Ichijo H, Hamazaki J, Murata S.  The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction  Elife  2016  5:e18357  PubMed ID: 27528193
13474  Kume K, Ikeda M, Miura S, Ito K, Sato KA, Ohmori Y, Endo F, Katagiri H, Ishida K, Ito C, Iwaya T, Nishizuka SS.  α-Amanitin Restrains Cancer Relapse from Drug-Tolerant Cell Subpopulations via TAF15  Sci Rep  2016  6:25895  PubMed ID: 27181033
7305  Yamada K, Maishi N, Akiyama K, Towfik Alam M, Ohga N, Kawamoto T, Shindoh M, Takahashi N, Kamiyama T, Hida Y, Taketomi A, Hida K.  CXCL12-CXCR7 axis is important for tumor endothelial cell angiogenic property.  Int. J. Cancer  2015  137:2825-36  PubMed ID: 26100110
7560  Shimodaira S, Hirabayashi K, Koya T, Higuchi Y, Sano K  Standard WT1 Expression Using Immunohistochemistry.  J Mol Biomark Diagn  2015  7:266 
4397  Pan J, Zhong J, Gan LH, Chen SJ, Jin HC, Wang X, Wang LJ.  Klotho, an anti-senescence related gene, is frequently inactivated through promoter hypermethylation in colorectal cancer.  Tumour Biol  2011  32(4):729-35  PubMed ID: 21523445



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