Cell No. : Cell Name
RCB2945 : DIT29
update : 2024/08/14
|
Comment | Medaka cell line derived from hepatoma cells. |
Comment from the depositor | |
Terms and conditions | 1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Fac Sci Univ Tokyo IV 1980;14:391-398 ) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
Basic information
|
Depositor |
Mitani, Hiroshi
|
Originator |
Mitani, Hiroshi
|
Year of deposit |
2009
|
Animal |
_medaka
< Fishes
|
Genus |
Oryzias
|
Species |
latipes
|
Tissue |
liver
|
Disease name |
hepatoma
|
Classification |
cancer
|
|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_J028
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
L-15 + 10mM HEPES + 20% FBS
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
Culture information
|
Passage ratio |
|
1 : 6-8 split
|
SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
Temperature |
|
33
℃
|
CO2 concentration |
|
0
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Images |
deposit info | lot info |
|
|
Reference information |
Reference |
1
|
User's Publication |
3
|
User's Publication |
20676
Tran NT, Vo LK, Komatsu M, Shiozaki K.
Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity.
Fish Shellfish Immunol
2022
124:534-542
PubMed ID: 35477099
DOI: 10.1016/j.fsi.2022.04.033
|
15275
Oishi K, Morise M, Vo LK, Tran NT, Sahashi D, Ueda-Wakamatsu R, Nishimura W, Komatsu M, Shiozaki K.
Host lactosylceramide enhances Edwardsiella tarda infection
Cell Microbiol
2021
23(9):e13365
PubMed ID: 33988901
DOI: 10.1111/cmi.13365
|
12624
Maekawa S, Chiang YA, Hikima J, Sakai M, Lo CF, Wang HC, Aoki T.
Expression and biological activity of two types of interferon genes in medaka (Oryzias latipes)
Fish Shellfish Immunol
2016
48:20-9
PubMed ID: 26655956
DOI: 10.1016/j.fsi.2015.11.036
|