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Cell No. : Cell Name
RCB2944 : OLCAB-e31  update : 2023/10/16
CommentMedaka cell line.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Comp Physiol B 2006;176:311-320) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mitani, Hiroshi
Originator Mitani, Hiroshi
Year of deposit 2009
Animal _medaka < Fishes
Genus Oryzias
Species latipes
Tissue embryo
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_M757
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium L-15 + 10mM HEPES + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 6-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 2
User's Publication 0

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10689  Kei Adachi, Kosuke Sumiyoshi, Ryo Ariyasu, Kasumi Yamashita, Kosuke Zenke, Yasushi Okinaka  Susceptibilities of Medaka (Oryzias Latipes) Cell Lines to a Betanodavirus  Virol J  2010  7:150  PubMed ID: 20624282   DOI: 10.1186/1743-422X-7-150
3634  Hirayama M, Mitani H, Watabe S.  Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations.  J Comp Physiol B  2006  176(4):311-20  PubMed ID: 16341864   DOI: 10.1007/s00360-005-0053-8

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