Cell No. : Cell Name
RCB2942 : OLHNI-2
update : 2023/10/16
|
| Comment | Medaka cell line derived from HNI strain. |
| Comment from the depositor | |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Comp Physiol B 2006;176:311-320) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Mitani, Hiroshi
|
| Originator |
Mitani, Hiroshi
|
| Year of deposit |
2009
|
| Animal |
_medaka
< Fishes
|
| Genus |
Oryzias
|
| Species |
latipes
|
| Tissue |
caudal fin
|
| Classification |
other
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_J027
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
L-15 + 10mM HEPES + 20% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
|
Passage ratio |
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1 : 6-8 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
0
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Images |
deposit info | lot info |
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|
| Reference information |
Reference |
2
|
| User's Publication |
2
|
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