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Cell No. : Cell Name
RCB2942 : OLHNI-2  update : 2018/07/06
CommentMedaka cell line derived from HNI strain.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Comp Physiol B 2006;176:311-320) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Mitani, Hiroshi
Originator Mitani, Hiroshi
Year of deposit 2009
Animal medaka < Fishes
Genus Oryzias
Species latipes
Tissue caudal fin
Classification other
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives L-15 + 10mM HEPES + 20% FBS
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 6-8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 2
User's Publication 1

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10689  Kei Adachi, Kosuke Sumiyoshi, Ryo Ariyasu, Kasumi Yamashita, Kosuke Zenke, Yasushi Okinaka  Susceptibilities of Medaka (Oryzias Latipes) Cell Lines to a Betanodavirus  Virol J  2020  7:150  PubMed ID: 20624282
3634  Hirayama M, Mitani H, Watabe S.  Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations.  J Comp Physiol B  2006  176(4):311-20  PubMed ID: 16341864

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User's Publication
9566  Ogiwara K, Takahashi T.  A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells.  Biol. Reprod.  2016  94:64  PubMed ID: 26864196

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