Cell No. : Cell Name
RCB2932 : MEDEP-BRC4-2
update : 2024/01/17
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| Comment | Mouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC4 (AES0008). SCF (Stem cell factor) and Dexamethasone dependent. C57BL/6 strain. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hiroyama, Takashi
|
| Originator |
Hiroyama, Takashi
|
| Year of deposit |
2009
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6
|
| Gender |
Unknown
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells : BRC4(RCB2008)
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| Classification |
other
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_E136
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10 -6M Dexamethasone + 50μg/ml Ascorbic acid + 50ng/ml mouse SCF
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 4-6 split
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| SC frequency |
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Subculture : 2-3 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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