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Cell No. : Cell Name
RCB2931 : 6E2  update : 2021/05/20
CommentHybridoma cell line expressing anti-human SSEA-4 monoclonal antibody.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hata, Jun-ichi
Originator Hata, Jun-ichi
Year of deposit 2009
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KA97
hybridoma Fusion_cell_1 name myeloma
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse
Antibody name anti-SSEA-4
Subclass IgG3
Immunogen human EC cell line (RCB2343 NCR-G3)
Antigen_name SSEA-4
Antigen_m.w. 81kDa
Antigen_character EC cell
Antigen_positive ES cell, EC cell, testis, ovary
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-12 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
4439  Ban Sato, Yohko U Katagiri, Kenji Miyado, Hidenori Akutsu, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hideki Nakajima, Hajime Okita, Akihiro Umezawa, Jun-Ichi Hata, Junichiro Fujimoto, Kiyotaka Toshimori, Nobutaka Kiyokawa  Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos  Biochem Biophys Res Commun  2007  364(4):838-43  PubMed ID: 18273440
4403  T Nakano, A Umezawa, H Abe, N Suzuki, T Yamada, S Nozawa, J Hata  A monoclonal antibody that specifically reacts with human embryonal carcinomas, spermatogonia and oocytes is able to induce human EC cell death  Differentiation  1995  58(3):233-40  PubMed ID: 7713330

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