Cell No. : Cell Name
RCB2931 : 6E2
update : 2024/01/17
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| Comment | Hybridoma cell line expressing anti-human SSEA-4 monoclonal antibody. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Hata, Jun-ichi
|
| Originator |
Hata, Jun-ichi
|
| Year of deposit |
2009
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_KA97
|
|
hybridoma
|
Fusion_cell_1 name |
myeloma
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen
|
| Fusion_cell_2 strain |
mouse
|
|
Antibody name |
anti-SSEA-4
|
| Subclass |
IgG3
|
| Immunogen |
human EC cell line (RCB2343 NCR-G3)
|
|
Antigen_name |
SSEA-4
|
| Antigen_m.w. |
81kDa
|
| Antigen_character |
EC cell
|
|
Antigen_positive |
ES cell, EC cell, testis, ovary
|
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10-12 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Antibody subclass |
|
IgG3
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
4439
Ban Sato, Yohko U Katagiri, Kenji Miyado, Hidenori Akutsu, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hideki Nakajima, Hajime Okita, Akihiro Umezawa, Jun-Ichi Hata, Junichiro Fujimoto, Kiyotaka Toshimori, Nobutaka Kiyokawa
Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos
Biochem Biophys Res Commun
2007
364(4):838-43
PubMed ID: 18273440
DOI: 10.1016/j.bbrc.2007.10.093
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4403
T Nakano, A Umezawa, H Abe, N Suzuki, T Yamada, S Nozawa, J Hata
A monoclonal antibody that specifically reacts with human embryonal carcinomas, spermatogonia and oocytes is able to induce human EC cell death
Differentiation
1995
58(3):233-40
PubMed ID: 7713330
DOI: 10.1046/j.1432-0436.1995.5830233.x
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