細胞番号 : 細胞名
RCB2927 : OP9/N-DLL1
update : 2024/11/11
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| 細胞特性(Comment:英) | Subline of OP9 cell line, expressing DLL1 and human NGF receptor. See RCB2120 TSt-4/N-DLL1. |
| 細胞特性(日) | OP9亜株。DLL1とヒトNGF受容体を発現。RCB2120TSt-4/N-DLL1参照。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com). |
| 使用条件(日) | 学術研究における利用につきましては原則として使用制限はありません。特許等の取得及び商業用途（商業利用）の使用については、リバーセル株式会社(info@rebirthel.com)に問い合わせてください。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Kawamoto, Hiroshi
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| 樹立者 |
Kawamoto, Hiroshi
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| 寄託日 |
2009
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
B6C3Fe-a/a, op
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| 性別 |
Unknown
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| 齢 |
0 day
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| 採取組織 |
newborn, calvaria
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| 遺伝的特徴 |
op/op
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| 細胞分類 |
transformed
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1)
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|
細胞寿命 |
infinite
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| 細胞形態 |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_B220
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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MEM ALPHA + 20% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture or Medium Renewal : once/2 days
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| マイコプラズマ |
(-)
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| マウス系統検査 |
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OK
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| アイソザイム検査 |
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LD, NP
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
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| Reference(日) |
0件
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| 利用者成果(英) |
10件
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| 利用者成果(日) |
0件
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| Reference(英) |
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12338
Raul Vizcardo, Kyoko Masuda, Daisuke Yamada, Tomokatsu Ikawa, Kanako Shimizu, Shin-Ichiro Fujii, Haruhiko Koseki, Hiroshi Kawamoto
Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells
Cell Stem Cell
2013
12(1):31-6
PubMed ID: 23290135
DOI: 10.1016/j.stem.2012.12.006
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3312
Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto
Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.
J Immunol
2005
174:2507-16
PubMed ID: 15728456
DOI: 10.4049/jimmunol.174.5.2507
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| 利用者成果(英) |
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19957
Itoh M, Kawagoe S, Nakagawa H, Asahina A, Okano HJ.
Generation of induced pluripotent stem cell (iPSC) from NY-ESO-I-specific cytotoxic T cells isolated from the melanoma patient with minor HLAs: The practical pilot study for the adoptive immunotherapy for melanoma using iPSC technology
Exp Dermatol
2023
32(2):126-134
PubMed ID: 36222007
DOI: 10.1111/exd.14686
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21206
Tamaoki N, Siebert S, Maeda T, Ha NH, Good ML, Huang Y, Vodnala SK, Haro-Mora JJ, Uchida N, Tisdale JF, Sweeney CL, Choi U, Brault J, Koontz S, Malech HL, Yamazaki Y, Isonaka R, Goldstein DS, Kimura M, Takebe T, Zou J, Stroncek DF, Robey PG, Kruhlak MJ, Restifo NP, Vizcardo R.
Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from induced pluripotent stem cells.
Cell Rep Methods
2023
3(4):100460
PubMed ID: 37159663
DOI: 10.1016/j.crmeth.2023.100460
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11382
Nishimura T, Nakauchi H.
Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells.
Methods Mol. Biol.
2019
PubMed ID: 30649763
DOI: 10.1007/978-1-4939-8938-6_3
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14056
Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP.
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
J Vis Exp
2019
(150)
PubMed ID: 31449236
DOI: 10.3791/58672
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14812
Good ML, Vizcardo R, Maeda T, Tamaoki N, Malekzadeh P, Kawamoto H, Restifo NP.
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
J Vis Exp
2019
(152)
PubMed ID: 31710026
DOI: 10.3791/59997
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10966
Yamada D, Iyoda T, Shimizu K, Sato Y, Koseki H, Fujii SI.
Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24+iNKT Cells
BioProtoc
2017
7
PubMed ID: 34541057
DOI: 10.21769/BioProtoc.2277
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7053
Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.
Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.
Cancer Res.
2016
76:6839-6850
PubMed ID: 27872100
DOI: 10.1158/0008-5472.CAN-16-1149
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18709
Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H.
Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation
Cell Stem Cell
2013
12(1):114-26
PubMed ID: 23290140
DOI: 10.1016/j.stem.2012.11.002
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19069
Satoh Y, Yokota T, Sudo T, Kondo M, Lai A, Kincade PW, Kouro T, Iida R, Kokame K, Miyata T, Habuchi Y, Matsui K, Tanaka H, Matsumura I, Oritani K, Kohwi-Shigematsu T, Kanakura Y.
The Satb1 protein directs hematopoietic stem cell differentiation toward lymphoid lineages
Immunity
2013
38(6):1105-15
PubMed ID: 23791645
DOI: 10.1016/j.immuni.2013.05.014
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6113
Eyrich M, Schreiber SC, Wollny G, Ziegler H, Schlenker R, Koch-Büttner K, Wölfl M, Schlegel PG, Schilbach K. Author information 1 Stem Cell Laboratory, University Children's Hospital Würzburg, Würzburg, Germany. Eyrich_m@klinik.uni-wuerzburg.de Abstract T-cell re-constitution after allogeneic stem cell transplantation (alloSCT) is often dampened by the slow differentiation of human peripheral blood CD34(+) (huCD34(+) ) hematopoietic stem cells (HSCs) into mature T cells. This process may be accelerated by the co-transfer of in vitro-pre-differentiated committed T/NK-lymphoid progenitors (CTLPs). Here, we analysed the developmental potential of huCD34(+) HSCs compared with CTLPs from a third-party donor in a murine NOD-scid IL2Rγ(null) model of humanised chimeric haematopoiesis. CTLPs (CD34(+) lin(-) CD45RA(+) CD7(+) ) could be generated in vitro within 10 days upon co-culture of huCD34(+) or cord blood CD34(+) (CB-CD34) HSCs on murine OP9/N-DLL-1 stroma cells but not in a novel 3-D cell-culture matrix with DLL-1(low) human stroma cells. In both in vitro systems, huCD34(+) and CB-CD34(+) HSCs did not give rise to mature T cells. Upon transfer into 6-wk-old immune-deficient mice, CTLPs alone did not engraft. However, transplantation of CTLPs together with huCD34(+) HSCs resulted in rapid T-cell engraftment in spleen, bone marrow and thymus at day 28. Strikingly, at this early time point mature T cells originated exclusively from CTLPs, whereas descendants of huCD34(+) HSCs still expressed a T-cell-precursor phenotype (CD7(+) CD5(+) CD1a(+/-) ). This strategy to enhance early T-cell re-constitution with ex vivo-pre-differentiated T-lymphoid progenitors could bridge the gap until full T-cell recovery in severely immunocompromised patients after allogeneic stem cell transplantation. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. PMID: 21928276 DOI: 10.1002/eji.201141561 [Indexed for MEDLINE] Free full text Share on FacebookShare on TwitterShare on Google+ Publication type, MeSH terms LinkOut - more resources Supplemental Content Full text links Icon for Wiley Save items View more options Similar articles Charac
Pre-differentiated human committed T-lymphoid progenitors promote peripheral T-cell re-constitution after stem cell transplantation in immunodeficient mice.
Eur J Immunol
2011
41(12):3596-603
PubMed ID: 21928276
DOI: 10.1002/eji.201141561
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