Cell No. : Cell Name
RCB2927 : OP9/N-DLL1
update : 2022/12/21
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Comment | Subline of OP9 cell line, expressing DLL1 and human NGF receptor. See RCB2120 TSt-4/N-DLL1. |
Comment from the depositor | |
Terms and conditions | Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com). |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Kawamoto, Hiroshi
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Originator |
Kawamoto, Hiroshi
|
Year of deposit |
2009
|
Animal |
_mouse
< Mammals
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Strain name |
B6C3Fe-a/a, op
|
Gender |
Unknown
|
Age at sampling |
0 day
|
Tissue |
newborn, calvaria
|
Genetics |
op/op
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Classification |
transformed
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Recombinant |
recombinant
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Exogene |
MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1)
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|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_B220
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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MEM ALPHA + 20% FBS
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Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture or Medium Renewal : once/2 days
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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SSLP(mouse) |
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OK
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Isozyme |
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LD, NP
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
|
User's Publication |
7
|
Reference |
12338
Raul Vizcardo, Kyoko Masuda, Daisuke Yamada, Tomokatsu Ikawa, Kanako Shimizu, Shin-Ichiro Fujii, Haruhiko Koseki, Hiroshi Kawamoto
Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells
Cell Stem Cell
2013
12(1):31-6
PubMed ID: 23290135
DOI: 10.1016/j.stem.2012.12.006
|
3312
Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto
Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.
J Immunol
2005
174:2507-16
PubMed ID: 15728456
DOI: 10.4049/jimmunol.174.5.2507
|
User's Publication |
21206
Tamaoki N, Siebert S, Maeda T, Ha NH, Good ML, Huang Y, Vodnala SK, Haro-Mora JJ, Uchida N, Tisdale JF, Sweeney CL, Choi U, Brault J, Koontz S, Malech HL, Yamazaki Y, Isonaka R, Goldstein DS, Kimura M, Takebe T, Zou J, Stroncek DF, Robey PG, Kruhlak MJ, Restifo NP, Vizcardo R.
Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from induced pluripotent stem cells.
Cell Rep Methods
2023
3(4):100460
PubMed ID: 37159663
DOI: 10.1016/j.crmeth.2023.100460
|
11382
Nishimura T, Nakauchi H.
Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells.
Methods Mol. Biol.
2019
PubMed ID: 30649763
DOI: 10.1007/978-1-4939-8938-6_3
|
14056
Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP.
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
J Vis Exp
2019
(150)
PubMed ID: 31449236
DOI: 10.3791/58672
|
14812
Good ML, Vizcardo R, Maeda T, Tamaoki N, Malekzadeh P, Kawamoto H, Restifo NP.
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
J Vis Exp
2019
(152)
PubMed ID: 31710026
DOI: 10.3791/59997
|
10966
Yamada D, Iyoda T, Shimizu K, Sato Y, Koseki H, Fujii SI.
Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24+iNKT Cells
BioProtoc
2017
7
PubMed ID: 34541057
DOI: 10.21769/BioProtoc.2277
|
7053
Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.
Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.
Cancer Res.
2016
76:6839-6850
PubMed ID: 27872100
DOI: 10.1158/0008-5472.CAN-16-1149
|
6113
Eyrich M, Schreiber SC, Wollny G, Ziegler H, Schlenker R, Koch-Büttner K, Wölfl M, Schlegel PG, Schilbach K. Author information 1 Stem Cell Laboratory, University Children's Hospital Würzburg, Würzburg, Germany. Eyrich_m@klinik.uni-wuerzburg.de Abstract T-cell re-constitution after allogeneic stem cell transplantation (alloSCT) is often dampened by the slow differentiation of human peripheral blood CD34(+) (huCD34(+) ) hematopoietic stem cells (HSCs) into mature T cells. This process may be accelerated by the co-transfer of in vitro-pre-differentiated committed T/NK-lymphoid progenitors (CTLPs). Here, we analysed the developmental potential of huCD34(+) HSCs compared with CTLPs from a third-party donor in a murine NOD-scid IL2Rγ(null) model of humanised chimeric haematopoiesis. CTLPs (CD34(+) lin(-) CD45RA(+) CD7(+) ) could be generated in vitro within 10 days upon co-culture of huCD34(+) or cord blood CD34(+) (CB-CD34) HSCs on murine OP9/N-DLL-1 stroma cells but not in a novel 3-D cell-culture matrix with DLL-1(low) human stroma cells. In both in vitro systems, huCD34(+) and CB-CD34(+) HSCs did not give rise to mature T cells. Upon transfer into 6-wk-old immune-deficient mice, CTLPs alone did not engraft. However, transplantation of CTLPs together with huCD34(+) HSCs resulted in rapid T-cell engraftment in spleen, bone marrow and thymus at day 28. Strikingly, at this early time point mature T cells originated exclusively from CTLPs, whereas descendants of huCD34(+) HSCs still expressed a T-cell-precursor phenotype (CD7(+) CD5(+) CD1a(+/-) ). This strategy to enhance early T-cell re-constitution with ex vivo-pre-differentiated T-lymphoid progenitors could bridge the gap until full T-cell recovery in severely immunocompromised patients after allogeneic stem cell transplantation. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. PMID: 21928276 DOI: 10.1002/eji.201141561 [Indexed for MEDLINE] Free full text Share on FacebookShare on TwitterShare on Google+ Publication type, MeSH terms LinkOut - more resources Supplemental Content Full text links Icon for Wiley Save items View more options Similar articles Charac
Pre-differentiated human committed T-lymphoid progenitors promote peripheral T-cell re-constitution after stem cell transplantation in immunodeficient mice.
Eur J Immunol
2011
41(12):3596-603
PubMed ID: 21928276
DOI: 10.1002/eji.201141561
|