Cell No. : Cell Name
RCB2927 : OP9/N-DLL1 update : 2021/02/02 |
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Comment | Subline of OP9 cell line, expressing DLL1 and human NGF receptor. See RCB2120 TSt-4/N-DLL1. | |||
Comment from the depositor | ||||
Terms and conditions | Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com). | |||
Remarks | ||||
Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
Basic information | Depositor | Kawamoto, Hiroshi | ||
Originator | Kawamoto, Hiroshi | |||
Year of deposit | 2009 | |||
Animal | mouse < Mammals | |||
Strain name | B6C3Fe-a/a, op | |||
Gender | Unknown | |||
Age at sampling | 0 day | |||
Tissue | newborn, calvaria | |||
Genetics | op/op | |||
Classification | transformed | |||
Recombinant | recombinant | |||
Exogene | MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1) | Lifespan | infinite | |
Morphology | fibroblast-like | |||
Medium | Medium List | Culture type | Adherent cells | |
Medium and additives | MEM ALPHA + 20% FBS | |||
Antibiotics | Free | Passage method | 0.25% trypsin | Culture information | Passage ratio | 1 : 4 split |
SC frequency | Subculture or Medium Renewal : once/2 days | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
SSLP(mouse) | OK | |||
Isozyme | LD, NP | |||
Images | ||||
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Reference information | Reference | 2 | ||
User's Publication | 4 | |||