Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB2927 : OP9/N-DLL1  update : 2021/02/02
CommentSubline of OP9 cell line, expressing DLL1 and human NGF receptor. See RCB2120 TSt-4/N-DLL1.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com).
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kawamoto, Hiroshi
Originator Kawamoto, Hiroshi
Year of deposit 2009
Animal mouse < Mammals
Strain name B6C3Fe-a/a, op
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Genetics op/op
Classification transformed
Recombinant recombinant
Exogene MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 20% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 4


To topTop
Reference
12338  Raul Vizcardo, Kyoko Masuda, Daisuke Yamada, Tomokatsu Ikawa, Kanako Shimizu, Shin-Ichiro Fujii, Haruhiko Koseki, Hiroshi Kawamoto  Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells  Cell Stem Cell  2013  12(1):31-6  PubMed ID: 23290135
3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456

To topTop
User's Publication
11382  Nishimura T, Nakauchi H.  Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2019    PubMed ID: 30649763
10966    Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24+iNKT Cells  BioProtoc  2017  7 
7053  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
6113  Eyrich M, Schreiber SC, Wollny G, Ziegler H, Schlenker R, Koch-Büttner K, Wölfl M, Schlegel PG, Schilbach K. Author information 1 Stem Cell Laboratory, University Children's Hospital Würzburg, Würzburg, Germany. Eyrich_m@klinik.uni-wuerzburg.de Abstract T-cell re-constitution after allogeneic stem cell transplantation (alloSCT) is often dampened by the slow differentiation of human peripheral blood CD34(+) (huCD34(+) ) hematopoietic stem cells (HSCs) into mature T cells. This process may be accelerated by the co-transfer of in vitro-pre-differentiated committed T/NK-lymphoid progenitors (CTLPs). Here, we analysed the developmental potential of huCD34(+) HSCs compared with CTLPs from a third-party donor in a murine NOD-scid IL2Rγ(null) model of humanised chimeric haematopoiesis. CTLPs (CD34(+) lin(-) CD45RA(+) CD7(+) ) could be generated in vitro within 10 days upon co-culture of huCD34(+) or cord blood CD34(+) (CB-CD34) HSCs on murine OP9/N-DLL-1 stroma cells but not in a novel 3-D cell-culture matrix with DLL-1(low) human stroma cells. In both in vitro systems, huCD34(+) and CB-CD34(+) HSCs did not give rise to mature T cells. Upon transfer into 6-wk-old immune-deficient mice, CTLPs alone did not engraft. However, transplantation of CTLPs together with huCD34(+) HSCs resulted in rapid T-cell engraftment in spleen, bone marrow and thymus at day 28. Strikingly, at this early time point mature T cells originated exclusively from CTLPs, whereas descendants of huCD34(+) HSCs still expressed a T-cell-precursor phenotype (CD7(+) CD5(+) CD1a(+/-) ). This strategy to enhance early T-cell re-constitution with ex vivo-pre-differentiated T-lymphoid progenitors could bridge the gap until full T-cell recovery in severely immunocompromised patients after allogeneic stem cell transplantation. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. PMID: 21928276 DOI: 10.1002/eji.201141561 [Indexed for MEDLINE] Free full text Share on FacebookShare on TwitterShare on Google+ Publication type, MeSH terms LinkOut - more resources Supplemental Content Full text links Icon for Wiley Save items View more options Similar articles Charac  Pre-differentiated human committed T-lymphoid progenitors promote peripheral T-cell re-constitution after stem cell transplantation in immunodeficient mice.  Eur J Immunol  2011  41(12):3596-603  PubMed ID: 21928276



Back Back Return Top Page