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Cell No. : Cell Name
RCB2926 : OP9/N update : 2024/11/11 |
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| Comment | Subline of OP9 cell line, expressing human NGF receptor. See RCB2119 TSt-4/N. | |||
| Comment from the depositor | ||||
| Terms and conditions | Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com). | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Kawamoto, Hiroshi | ||
| Originator | Kawamoto, Hiroshi | |||
| Year of deposit | 2009 | |||
| Animal | _mouse < Mammals | |||
| Strain name | B6C3Fe-a/a, op | |||
| Gender | Unknown | |||
| Age at sampling | 0 day | |||
| Tissue | newborn, calvaria | |||
| Genetics | op/op | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | MSCV-IRES-NGF receptor(human) | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_B219 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | MEM ALPHA + 20% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 4 split |
| SC frequency | Subculture or Medium Renewal : once/2 days | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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