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Cell No. : Cell Name
RCB2925 : OP9/G-DLL1  update : 2023/10/16
CommentSubline of OP9 cell line, expressing DLL1 and EGFP. See RCB2118 TSt-4/G-DLL1.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kawamoto, Hiroshi
Originator Kawamoto, Hiroshi
Year of deposit 2009
Animal _mouse < Mammals
Strain name B6C3Fe-a/a, op
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Genetics op/op
Classification transformed
Recombinant recombinant
Exogene MSCV-IRES-EGFP,murine DLL1 (delta-like 1)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B218
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 4

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12338  Raul Vizcardo, Kyoko Masuda, Daisuke Yamada, Tomokatsu Ikawa, Kanako Shimizu, Shin-Ichiro Fujii, Haruhiko Koseki, Hiroshi Kawamoto  Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells  Cell Stem Cell  2013  12(1):31-6  PubMed ID: 23290135   DOI: 10.1016/j.stem.2012.12.006
3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456   DOI: 10.4049/jimmunol.174.5.2507

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User's Publication
16851  Tang SY, Zha S, Du Z, Zeng J, Zhu D, Luo Y, Wang S.  Targeted integration of EpCAM-specific CAR in human induced pluripotent stem cells and their differentiation into NK cells  Stem Cell Res Ther  2021  12(1):580  PubMed ID: 34802459   DOI: 10.1186/s13287-021-02648-4
17082  Oba T, Makino K, Kajihara R, Yokoi T, Araki R, Abe M, Minderman H, Chang AE, Odunsi K, Ito F.  In situ delivery of iPSC-derived dendritic cells with local radiotherapy generates systemic antitumor immunity and potentiates PD-L1 blockade in preclinical poorly immunogenic tumor models  J Immunother Cancer  2021  9(5):e002432  PubMed ID: 34049930   DOI: 10.1136/jitc-2021-002432
10754  Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.  Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis.  Exp. Cell Res.  2018  365:145-155  PubMed ID: 29458175   DOI: 10.1016/j.yexcr.2018.02.014
18709  Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H.  Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation  Cell Stem Cell  2013  12(1):114-26  PubMed ID: 23290140   DOI: 10.1016/j.stem.2012.11.002

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