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Cell No. : Cell Name
RCB2924 : OP9/G  update : 2022/04/18
CommentSubline of OP9 cell line, expressing EGFP. See RCB2117 TSt-4/G.
Comment from the depositor
Terms and conditions1) The RECIPIENT should read Announcement of GFP bioresources 2) Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com).
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawamoto, Hiroshi
Originator Kawamoto, Hiroshi
Year of deposit 2009
Animal mouse < Mammals
Strain name B6C3Fe-a/a, op
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Genetics op/op
Classification transformed
Recombinant recombinant
Exogene MSCV-IRES-EGFP
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B217
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456  

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User's Publication
10754  Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.  Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis.  Exp. Cell Res.  2018  365:145-155  PubMed ID: 29458175   DOI: 10.1016/j.yexcr.2018.02.014



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