Cell No. : Cell Name RCB2924 : OP9/G  update : 2024/11/11
Comment			Subline of OP9 cell line, expressing EGFP. See RCB2117 TSt-4/G.
Comment from the depositor
Terms and conditions			Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com).
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kawamoto, Hiroshi
		Originator	Kawamoto, Hiroshi
		Year of deposit	2009
		Animal	_mouse < Mammals
		Strain name	B6C3Fe-a/a, op
		Gender	Unknown
		Age at sampling	0 day
		Tissue	newborn, calvaria
		Genetics	op/op
		Classification	transformed
		Recombinant	recombinant
		Exogene	MSCV-IRES-EGFP
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_B217
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA + 20% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture or Medium Renewal : once/2 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Mycoplasma	(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	1

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Reference

3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456   DOI: 10.4049/jimmunol.174.5.2507

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User's Publication

10754  Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.  Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis.  Exp. Cell Res.  2018  365:145-155  PubMed ID: 29458175   DOI: 10.1016/j.yexcr.2018.02.014