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Cell No. : Cell Name
RCB2911 : MEDEP-BRC5  update : 2022/01/20
CommentMouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC5 (AES0009). SCF(Stem cell factor) dependent.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hiroyama, Takashi
Originator Hiroyama, Takashi
Year of deposit 2009
Another name B6EP-5(S)
Animal mouse < Mammals
Strain name C57BL/6
Gender Male
Age at sampling embryo
Tissue embryonic stem cells : BRC5(AES009)
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_E137
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10^(-6)M Dexamethasone + 50μg/ml Ascorbic acid + 50ng/ml mouse SCF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Others Thawing of frozen cells : spin at 1200rpm 3min.
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492

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User's Publication
13018  Kiatpakdee B, Sato K, Otsuka Y, Arashiki N, Chen Y, Tsumita T, Otsu W, Yamamoto A, Kawata R, Yamazaki J, Sugimoto Y, Takada K, Mohandas N, Inaba M.  Cholesterol-binding protein TSPO2 coordinates maturation and proliferation of terminally differentiating erythroblasts.  J Biol Chem  2020    PubMed ID: 32358067
11390  Yang C, Hashimoto M, Lin QXX, Tan DQ, Suda T.  Sphingosine-1-phosphate signaling modulates terminal erythroid differentiation through the regulation of mitophagy.  Exp. Hematol.  2019    PubMed ID: 30658118
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014 



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