Cell No. : Cell Name
RCB2911 : MEDEP-BRC5
update : 2024/08/14
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| Comment | Mouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC5 (AES0009). SCF(Stem cell factor) dependent. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hiroyama, Takashi
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| Originator |
Hiroyama, Takashi
|
| Year of deposit |
2009
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| Another name |
B6EP-5(S)
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| Animal |
_mouse
< Mammals
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| Strain name |
C57BL/6
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells : BRC5(AES009)
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| Classification |
other
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_E137
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10 -6M Dexamethasone + 50μg/ml Ascorbic acid + 50ng/ml mouse SCF
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 3 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Others |
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Thawing of frozen cells : spin at 1200rpm 3min.
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
3
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| User's Publication |
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13018
Kiatpakdee B, Sato K, Otsuka Y, Arashiki N, Chen Y, Tsumita T, Otsu W, Yamamoto A, Kawata R, Yamazaki J, Sugimoto Y, Takada K, Mohandas N, Inaba M.
Cholesterol-binding protein TSPO2 coordinates maturation and proliferation of terminally differentiating erythroblasts.
J Biol Chem
2020
PubMed ID: 32358067
DOI: 10.1074/jbc.RA119.011679
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11390
Yang C, Hashimoto M, Lin QXX, Tan DQ, Suda T.
Sphingosine-1-phosphate signaling modulates terminal erythroid differentiation through the regulation of mitophagy.
Exp. Hematol.
2019
PubMed ID: 30658118
DOI: 10.1016/j.exphem.2019.01.004
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8207
Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura
Development of a simple method to determine the mouse strain from which cultured cell lines originated
Interdisciplinary Bio Central
2010
2:0014
DOI: 10.4051/ibc.2010.2.4.0014
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