Cell No. : Cell Name
RCB2911 : MEDEP-BRC5 update : 2023/03/15 |
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Comment | Mouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC5 (AES0009). SCF(Stem cell factor) dependent. | |||
Comment from the depositor | ||||
Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Hiroyama, Takashi | ||
Originator | Hiroyama, Takashi | |||
Year of deposit | 2009 | |||
Another name | B6EP-5(S) | |||
Animal | mouse < Mammals | |||
Strain name | C57BL/6 | |||
Gender | Male | |||
Age at sampling | embryo | |||
Tissue | embryonic stem cells : BRC5(AES009) | |||
Classification | other | Lifespan | infinite | |
Morphology | lymphocyte-like | |||
Cellosaurus(Expasy) | CVCL_E137 | |||
Medium | Medium List | Culture type | Suspension cells | |
Culture medium | IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10^(-6)M Dexamethasone + 50μg/ml Ascorbic acid + 50ng/ml mouse SCF | |||
Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : 3 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
SSLP(mouse) | OK | |||
Isozyme | LD, NP | |||
Others | Thawing of frozen cells : spin at 1200rpm 3min. | |||
Images | ||||
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Reference information | Reference | 1 | ||
User's Publication | 3 | |||