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Cell No. : Cell Name
RCB2909 : Walker256-TC  update : 2021/05/20
CommentRat cell line derived from mammary carcinoma. TKG0565 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0565
Animal rat < Mammals
Strain name Wistar
Gender Female
Tissue mammary gland
Disease name mammary gland carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0565)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4984
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
deposit info
lot info
Reference information Reference 6
User's Publication 1

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5388  Arakawa M, Shimizu F, Sasagawa K, Inomata T, Shinkai K.  Antitumor activity of methyl (5-fluoro-1H-2-oxo-4-pyrimidinyl beta-D-glucopyranosid)uronate against various experimental tumors.  Gan  1981  72(2):220-5  PubMed ID: 6793441  
5389  Chen CH, Chen SC.  Cell growth factor activity. New quantitative method in cell culture assay.  Exp Cell Res  1981  136(1):43-51  PubMed ID: 7028494   DOI: 10.1016/0014-4827(81)90036-7
5390  Wass JA, Varani J, Piontek GE, Goff D, Ward PA.  Characteristics of the chemotactic factor-mediated cell swelling response of tumor cells.  J Natl Cancer Inst  1981  66(5):927-33  PubMed ID: 6939935  
5391  Goodlad GA, Clark CM.  Actin polymerisation in Walker 256 carcinoma cells from solid or ascitic tumours.  Cancer Lett  1981  13(2):129-32  PubMed ID: 7306949   DOI: 10.1016/0304-3835(81)90138-5
5387  DUNHAM LJ, STEWART HL.  A survey of transplantable and transmissible animal tumors.  J Natl Cancer Inst  1953  13:1299-377  PubMed ID: 13035452  
5386  WILTON R. EARLE   A study of the Walker rat mammary carcinoma 256, in vivo and in vitro  Am J Cancer  1935  24, 566-612    DOI: 10.1158/ajc.1935.566

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User's Publication
13438  Takayuki Kamanaka, Hisao Haniu, Manabu Tanaka, Takashi Takizawa, Kaoru Aoki, Masanori Okamoto, Atsushi Sobajima, Kazushige Yoshida, Hirokazu Ideta, Tetsuhiko Mimura, Haruka Ishida, Katsuya Ueda, Takeshi Uemura, Jin Hee Kim, Yoong Ahm Kim, Hiroyuki Katoa and Naoto Saito  Carbon fibers for treatment of cancer metastasis in bone  RSC Adv  2020      DOI: 10.1039/D0RA05992G

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