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Cell No. : Cell Name
RCB2908 : AH272-TC  update : 2024/01/17
CommentRat cell line derived from hepatoma cells in ascites. TKG0535 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0535
Animal _rat < Mammals
Strain name Donryu
Tissue liver, ascites
Disease name liver, ascites hepatoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0535)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_5241
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
deposit info
lot info
Reference information Reference 4
User's Publication 0

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5385  Suzuki M, Hori K, Abe I, Saito S, Sato H.  A new approach to cancer chemotherapy: selective enhancement of tumor blood flow with angiotensin II.  J Natl Cancer Inst  1981  67(3):663-9  PubMed ID: 6944536  
5384  Kawaguchi T, Kitamura H, Nakamura K.  Tumor formation of rat ascites hepatoma cells in the traumatized brain.  Gan  1979  70(3):337-42  PubMed ID: 467895  
5383  Kawaguchi T, Nakamura K.  Relationship between transcerebral passage of tumor cells and brain metastasis.  Gan  1977  68(1):65-71  PubMed ID: 193754  
5382  YOSHIDA T, ODASHIMA S, KURATA T, NAKAMURA K, IRAKO Y, ISAKA H, ISHIZAWA T.  Studies on the ascites hepatoma. XII. Report on establishment of nine further transplant strains.  Gan  1957  48(4):551-3  PubMed ID: 13524500  

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