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Cell No. : Cell Name
RCB2904 : WEHI164R  update : 2023/10/16
CommentMouse cell line derived from fibrosarcoma. TNF resistant. BALB/c strain. TKG0281 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J. Immunol., 141, 3422-328, 1988) designated by the DEPOSITOR is required.
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Basic information Depositor Ohta, N. (Fukuda, Hiroshi)
Originator Williamson, B.
Year of deposit 2008
Original cell TKG0281
Animal _mouse < Mammals
Strain name BALB/c
Tissue fibrosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0281)
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4561
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 0

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5290  Lattime EC, Stoppacciaro A, Stutman O.  Limiting dilution analysis of TNF producing cells in C3H/HeJ mice.  J Immunol  1988  15;141(10):3422-8  PubMed ID: 2903192  

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