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Cell No. : Cell Name
RCB2904 : WEHI164R  update : 2021/05/20
CommentMouse cell line derived from fibrosarcoma. TNF resistant. BALB/c strain. TKG0281 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J. Immunol., 141, 3422-328, 1988) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohta, N. (Fukuda, Hiroshi)
Originator Williamson, B.
Year of deposit 2008
Original cell TKG0281
Animal mouse < Mammals
Strain name BALB/c
Tissue fibrosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0281)
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4561
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5290  Lattime EC, Stoppacciaro A, Stutman O.  Limiting dilution analysis of TNF producing cells in C3H/HeJ mice.  J Immunol  1988  15;141(10):3422-8  PubMed ID: 2903192  

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