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Cell No. : Cell Name
RCB2901 : S180-NCC-TC  update : 2021/05/20
CommentMouse cell line derived from sarcoma cells in ascites. CFW strain. TKG0638 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Ikeda,M.
Year of deposit 2008
Original cell TKG0638
Animal mouse < Mammals
Strain name CFW
Tissue sarcoma, ascitic tumor
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0638)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_7891
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
5381  LEDLEY RS  ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.  Science  1964  146(3641):241-3  PubMed ID: 14185313
5380  FOLEY GE, DROLET BP, McCARTHY RE, GOULET KA, DOKOS JM, FILLER DA.  Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.  Cancer Res  1960  20:930-9  PubMed ID: 13823721
5379  FOLEY GE, DROLET BP.  Sustained propagation of sarcoma of 180 in tissue culture.  Proc Soc Exp Biol Med  1956  92(2):347-52  PubMed ID: 13350343

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