Cell No. : Cell Name RCB2899 : S180-Kumamoto-TC  update : 2023/10/16
Comment			Mouse cell line derived from Swiss Webster Sarcoma 180. CFW strain. TKG0637 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Ikeda,M
		Year of deposit	2008
		Original cell	TKG0637
		Animal	_mouse < Mammals
		Strain name	CFW
		Tissue	sarcoma, ascitic tumor
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0637）
		Lifespan	infinite
		Morphology	lymphoblast-like
		Cellosaurus(Expasy)	CVCL_7889
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent/Suspension cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		pipetting or 0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	2

Top

Reference

5381  LEDLEY RS  ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.  Science  1964  146(3641):241-3  PubMed ID: 14185313

5380  FOLEY GE, DROLET BP, McCARTHY RE, GOULET KA, DOKOS JM, FILLER DA.  Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.  Cancer Res  1960  20:930-9  PubMed ID: 13823721

5379  FOLEY GE, DROLET BP.  Sustained propagation of sarcoma of 180 in tissue culture.  Proc Soc Exp Biol Med  1956  92(2):347-52  PubMed ID: 13350343   DOI: 10.3181/00379727-92-22473

Top

User's Publication

19716  Umeda IO, Koike Y, Ogata M, Kaneko E, Hamamichi S, Uehara T, Moribe K, Arano Y, Takahashi T, Fujii H.  New liposome-radionuclide-chelate combination for tumor targeting and rapid healthy tissue clearance  J Control Release  2023  847-855  PubMed ID: 37543291   DOI: 10.1016/j.jconrel.2023.07.060

14593  Yamamoto H, Saito S, Sawaguchi Y, Kimura M.  Identification of Protease Specificity Using Biotin-Labeled Substrates  Open Biochem J  2017  11:27-35  PubMed ID: 28567123   DOI: 10.2174/1874091X01711010027