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Cell No. : Cell Name
RCB2868 : NMuMG/Fucci2  update : 2024/07/16
CommentA subline of the NMuMG cell line expressing Fucci2, a cell cycle marker. Cell line name : NMuMG/Fucci2 #5. Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositor
Terms and conditions1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2009
Another name NMuMG-Fucci2
Original cell NMuMG
Animal _mouse < Mammals
Strain name NAMRU
Gender Female
Tissue mammary gland
Classification transformed
Recombinant recombinant
Exogene Fucci2
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U135
deposit info
lot info
Medium Medium List
Culture medium DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 7


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Reference
7987  Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A.  Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.  BMC Cell Biol.  2011  12:2  PubMed ID: 21226962   DOI: 10.1186/1471-2121-12-2

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User's Publication
20650  Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S.  Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.  Cells  2022  11(5)  PubMed ID: 35269503   DOI: 10.3390/cells11050881
20344  Narotamo H, Fernandes MS, Moreira AM, Melo S, Seruca R, Silveira M, Sanches JM.  A machine learning approach for single cell interphase cell cycle staging.  Sci Rep  2021  11(1):19278  PubMed ID: 34588507   DOI: 10.1038/s41598-021-98489-5
11547  Prakash V, Carson BB, Feenstra JM, Dass RA, Sekyrova P, Hoshino A, Petersen J, Guo Y, Parks MM, Kurylo CM, Batchelder JE, Haller K, Hashimoto A, Rundqivst H, Condeelis JS, Allis CD, Drygin D, Nieto MA, Andäng M, Percipalle P, Bergh J, Adameyko I, Farrants AÖ, Hartman J, Lyden D, Pietras K, Blanchard SC, Vincent CT.  Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease.  Nat Commun  2019    PubMed ID: 31068593   DOI: 10.1038/s41467-019-10100-8
11797  Lee DS, Luo C, Zhou J, Chandran S, Rivkin A, Bartlett A, Nery JR, Fitzpatrick C, O'Connor C, Dixon JR, Ecker JR.  Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.  Nat. Methods  2019    PubMed ID: 31501549   DOI: 10.1038/s41592-019-0547-z
11295  Yamazaki T, Taniguchi H, Tsuji S, Sato S, Kenmotsu T, Yoshikawa K, Sadakane K.  Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold.  J Vis Exp  2018    PubMed ID: 30417883   DOI: 10.3791/57815
10174  Ferro A, Mestre T, Carneiro P, Sahumbaiev I, Seruca R, Sanches JM.  Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images.  Lab. Invest.  2017  97:615-625  PubMed ID: 28263290   DOI: 10.1038/labinvest.2017.13
7381  Noguchi M, Kanari Y, Yokoya A, Narita A, Fujii K.  Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays.  Radiat Prot Dosimetry  2015  166:101-3  PubMed ID: 25883301   DOI: 10.1093/rpd/ncv157



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