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Cell No. : Cell Name
RCB2868 : NMuMG/Fucci2  update : 2020/08/18
CommentA subline of the NMuMG cell line expressing Fucci2, a cell cycle marker. Cell line name : NMuMG/Fucci2 #5. Important notice regarding nomenclature of Fucci.
Comment from the depositor
Terms and conditions1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2009
Another name NMuMG-Fucci2
Animal mouse < Mammals
Strain name NAMRU
Gender Female
Tissue mammary gland
Classification transformed
Recombinant recombinant
Exogene Fucci2
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
7987  Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A.  Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.  BMC Cell Biol.  2011  12:2  PubMed ID: 21226962

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User's Publication
11547  Prakash V, Carson BB, Feenstra JM, Dass RA, Sekyrova P, Hoshino A, Petersen J, Guo Y, Parks MM, Kurylo CM, Batchelder JE, Haller K, Hashimoto A, Rundqivst H, Condeelis JS, Allis CD, Drygin D, Nieto MA, Andäng M, Percipalle P, Bergh J, Adameyko I, Farrants AÖ, Hartman J, Lyden D, Pietras K, Blanchard SC, Vincent CT.  Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease.  Nat Commun  2019    PubMed ID: 31068593
11797  Lee DS, Luo C, Zhou J, Chandran S, Rivkin A, Bartlett A, Nery JR, Fitzpatrick C, O'Connor C, Dixon JR, Ecker JR.  Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.  Nat. Methods  2019    PubMed ID: 31501549
11295  Yamazaki T, Taniguchi H, Tsuji S, Sato S, Kenmotsu T, Yoshikawa K, Sadakane K.  Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold.  J Vis Exp  2018    PubMed ID: 30417883
10174  Ferro A, Mestre T, Carneiro P, Sahumbaiev I, Seruca R, Sanches JM.  Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images.  Lab. Invest.  2017  97:615-625  PubMed ID: 28263290
7381  Noguchi M, Kanari Y, Yokoya A, Narita A, Fujii K.  Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays.  Radiat Prot Dosimetry  2015  166:101-3  PubMed ID: 25883301



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