Cell No. : Cell Name
RCB2868 : NMuMG/Fucci2
update : 2025/01/15
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| Comment | A subline of the NMuMG cell line expressing Fucci2, a cell cycle marker. Cell line name : NMuMG/Fucci2 #5. Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | |
| Terms and conditions | 1)The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT should obtain written approval from the UCSD using the[mCherry FORM]. 3) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 4) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011). |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyawaki, Atsushi
|
| Originator |
Miyawaki, Atsushi
|
| Year of deposit |
2009
|
| Another name |
NMuMG-Fucci2
|
| Original cell |
NMuMG
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NAMRU
|
| Gender |
Female
|
| Tissue |
mammary gland
|
| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
Fucci2
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_U135
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deposit info |
lot info |
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Medium |
Medium List |
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Culture medium |
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DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
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| Antibiotics |
|
Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
6
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| User's Publication |
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20650
Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S.
Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.
Cells
2022
11(5)
PubMed ID: 35269503
DOI: 10.3390/cells11050881
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20344
Narotamo H, Fernandes MS, Moreira AM, Melo S, Seruca R, Silveira M, Sanches JM.
A machine learning approach for single cell interphase cell cycle staging.
Sci Rep
2021
11(1):19278
PubMed ID: 34588507
DOI: 10.1038/s41598-021-98489-5
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11547
Prakash V, Carson BB, Feenstra JM, Dass RA, Sekyrova P, Hoshino A, Petersen J, Guo Y, Parks MM, Kurylo CM, Batchelder JE, Haller K, Hashimoto A, Rundqivst H, Condeelis JS, Allis CD, Drygin D, Nieto MA, Andäng M, Percipalle P, Bergh J, Adameyko I, Farrants AÖ, Hartman J, Lyden D, Pietras K, Blanchard SC, Vincent CT.
Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease.
Nat Commun
2019
PubMed ID: 31068593
DOI: 10.1038/s41467-019-10100-8
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11797
Lee DS, Luo C, Zhou J, Chandran S, Rivkin A, Bartlett A, Nery JR, Fitzpatrick C, O'Connor C, Dixon JR, Ecker JR.
Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.
Nat. Methods
2019
PubMed ID: 31501549
DOI: 10.1038/s41592-019-0547-z
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10174
Ferro A, Mestre T, Carneiro P, Sahumbaiev I, Seruca R, Sanches JM.
Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images.
Lab. Invest.
2017
97:615-625
PubMed ID: 28263290
DOI: 10.1038/labinvest.2017.13
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7381
Noguchi M, Kanari Y, Yokoya A, Narita A, Fujii K.
Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays.
Radiat Prot Dosimetry
2015
166:101-3
PubMed ID: 25883301
DOI: 10.1093/rpd/ncv157
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