細胞番号 : 細胞名
RCB2867 : HeLa/Fucci2
update : 2023/10/16
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| 細胞特性(Comment:英) | A subline of the HeLa cell line expressing Fucci2, a cell cycle marker. Cell line name : HeLa/Fucci2 #3. Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| 細胞特性(日) | HeLa細胞亜株。細胞周期マーカーであるFucci2を発現している。株化細胞名：HeLa/Fucci2 #3。Important notice regarding nomenclature of Fucci。リサーチツール : 細胞周期インジケーター Fucci。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE，a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR. |
| 使用条件(日) | 英文に準拠、生物遺伝資源提供同意書 (MTA) には、英文をご記載またはご添付ください。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Miyawaki, Atsushi
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| 樹立者 |
Miyawaki, Atsushi
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| 寄託日 |
2009
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| 別名 |
HeLa.S-Fucci2
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| 元の細胞 |
HeLa.S3
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Female
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| 採取組織 |
cervix
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| 病名 |
cervical carcinoma
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| 細胞分類 |
cancer
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
Fucci2
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細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_U187
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM (low glucose) + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
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| Reference(日) |
0件
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| 利用者成果(英) |
18件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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17619
Ebata H, Umeda K, Nishizawa K, Nagao W, Inokuchi S, Sugino Y, Miyamoto T, Mizuno D.
Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology
Biophys J
2023
;S0006-3495(23)00241-2
PubMed ID: 37050875
DOI: 10.1016/j.bpj.2023.04.011
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21373
Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.
TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.
Nat Commun
2023
14(1):4521
PubMed ID: 37607907
DOI: 10.1038/s41467-023-40243-8
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21428
Honda K, Hitora Y, Tsukamoto S.
Akanthomins A-C, aphidicolin analogs from a fungus Akanthomyces sp., that inhibit cell cycle.
Phytochemistry
2023
216:113885
PubMed ID: 37806468
DOI: 10.1016/j.phytochem.2023.113885
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21584
Kazama R, Sakai S.
Effect of cell adhesiveness of Cell Dome shell on enclosed HeLa cells.
J Biosci Bioeng
2023
PubMed ID: 38307767
DOI: 10.1016/j.jbiosc.2024.01.002
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20650
Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S.
Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.
Cells
2022
11(5)
PubMed ID: 35269503
DOI: 10.3390/cells11050881
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13586
Hitora Y, Sejiyama A, Honda K, Ise Y, Losung F, Mangindaan REP, Tsukamoto S.
Fluorescent image-based high-content screening of extracts of natural resources for cell cycle inhibitors and identification of a new sesquiterpene quinone from the sponge, Dactylospongia metachromia
Bioorg Med Chem
2021
31:115968
PubMed ID: 33387695
DOI: 10.1016/j.bmc.2020.115968
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13202
Iwata H, Shuto T, Kamei S, Omachi K, Moriuchi M, Omachi C, Toshito T, Hashimoto S, Nakajima K, Sugie C, Ogino H, Kai H, Shibamoto Y.
Combined effects of cisplatin and photon or proton irradiation in cultured cells: radiosensitization, patterns of cell death and cell cycle distribution.
J Radiat Res
2020
PubMed ID: 32880637
DOI: 10.1093/jrr/rraa065
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13217
Yamanaka R, Shindo Y, Hotta K, Hiroi N, Oka K.
Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature.
Biochem Biophys Res Commun
2020
PubMed ID: 32928506
DOI: 10.1016/j.bbrc.2020.08.110
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13333
Kim H, Watanabe S, Kitamatsu M, Watanabe K, Ohtsuki T.
Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate.
Sci Rep
2020
PubMed ID: 33154435
DOI: 10.1038/s41598-020-76100-7
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14818
Shu S, Iimori M, Wakasa T, Ando K, Saeki H, Oda Y, Oki E, Maehara Y.
The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells
J Cell Sci
2019
132(24):jcs231530
PubMed ID: 31757888
DOI: 10.1242/jcs.231530
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4211
Panagiotakopoulou M, Lendenmann T, Pramotton FM, Giampietro C, Stefopoulos G, Poulikakos D, Ferrari A.
Cell cycle-dependent force transmission in cancer cells.
Mol. Biol. Cell
2018
PubMed ID: 30113874
DOI: 10.1091/mbc.E17-12-0726
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7021
Sasaki T, Sato Y, Higashiyama T, Sasaki N.
Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells.
Sci Rep
2017
7:11257
PubMed ID: 28900194
DOI: 10.1038/s41598-017-10843-8
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10632
Tsuda Y, Iimori M, Nakashima Y, Nakanishi R, Ando K, Ohgaki K, Kitao H, Saeki H, Oki E, Maehara Y.
Mitotic slippage and the subsequent cell fates after inhibition of Aurora B during tubulin-binding agent-induced mitotic arrest.
Sci Rep
2017
7:16762
PubMed ID: 29196757
DOI: 10.1038/s41598-017-17002-z
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10910
Intracellular calcium dynamics and expression of P2Y and IP3 receptors in a cycling G1-phase cell
Bioimages
2016
24 : 13-29
DOI: 10.11169/bioimages.24.13
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13989
Asahina T, Kaida A, Goto T, Yoshimura R, Sasai K, Miura M.
Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading system (HDR-RALS)
Radiat Oncol
2016
11:99
PubMed ID: 27473168
DOI: 10.1186/s13014-016-0669-8
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15170
Gao B, Konno T, Ishihara K.
Fabrication of a live cell-containing multilayered polymer hydrogel membrane with micrometer-scale thickness to evaluate pharmaceutical activity
J Biomater Sci Polym Ed
2015
26(18):1372-85
PubMed ID: 26374190
DOI: 10.1080/09205063.2015.1095025
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15171
Botao Gao, Tomohiro Konno, Kazuhiko Ishihara
Building cell-containing multilayered phospholipid polymer hydrogels for controlling diffusion of bioactive reagent
RSC Adv
2015
5:44408-44415
DOI: 10.1039/C5RA05299H
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16000
Murakami T, Aida Y.
Visualizing Vpr-induced G2 arrest and apoptosis
PLoS One
2014
9(1):e86840
PubMed ID: 24466265
DOI: 10.1371/journal.pone.0086840
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