細胞番号 : 細胞名 RCB2867 : HeLa/Fucci2  update : 2024/07/16
細胞特性(Comment:英)			A subline of the HeLa cell line expressing Fucci2, a cell cycle marker. Cell line name : HeLa/Fucci2 #3. Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
細胞特性(日)			HeLa細胞亜株。細胞周期マーカーであるFucci2を発現している。株化細胞名：HeLa/Fucci2 #3。Important notice regarding nomenclature of Fucci。リサーチツール :Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the　BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE，a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
使用条件(日)			英文に準拠、生物遺伝資源提供同意書 (MTA) には、英文をご記載またはご添付ください。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Miyawaki, Atsushi
		樹立者	Miyawaki, Atsushi
		寄託日	2009
		別名	HeLa.S-Fucci2
		元の細胞	HeLa.S3
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		性別	Female
		採取組織	cervix
		病名	cervical carcinoma
		細胞分類	cancer
		遺伝子改変	recombinant
		外来遺伝子	Fucci2
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_U187
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		DMEM (low glucose) + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		アイソザイム検査		LD, NP
		個体識別検査		OK
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	2件
		Reference(日)	0件
		利用者成果(英)	20件
		利用者成果(日)	0件

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Reference(英)

7987  Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A.  Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.  BMC Cell Biol.  2011  12:2  PubMed ID: 21226962   DOI: 10.1186/1471-2121-12-2

150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265

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Reference(日)

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利用者成果(英)

21584  Kazama R, Sakai S.  Effect of cell adhesiveness of Cell Dome shell on enclosed HeLa cells.  J Biosci Bioeng  2024  137(4):313-320  PubMed ID: 38307767   DOI: 10.1016/j.jbiosc.2024.01.002

21947  Mubarok W, Elvitigala KCML, Nakaya H, Hotta T, Sakai S.  Cell Cycle Modulation through Physical Confinement in Micrometer-Thick Hydrogel Sheaths.  Langmuir  2024    PubMed ID: 39166379   DOI: 10.1021/acs.langmuir.4c02434

22129  Iwata H, Toshito T, Omachi C, Umezawa M, Yamada M, Tanaka K, Nakajima K, Tsuzuki Y, Matsumoto K, Kawai T, Shibata Y, Ugawa S, Ogino H, Hiwatashi A.  Proton FLASH Irradiation Using a Synchrotron Accelerator: Differences by Irradiation Positions.  Int J Radiat Oncol Biol Phys  2024    PubMed ID: 39549758   DOI: 10.1016/j.ijrobp.2024.11.066

17619  Ebata H, Umeda K, Nishizawa K, Nagao W, Inokuchi S, Sugino Y, Miyamoto T, Mizuno D.  Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology  Biophys J  2023  ;S0006-3495(23)00241-2  PubMed ID: 37050875   DOI: 10.1016/j.bpj.2023.04.011

21373  Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.  TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.  Nat Commun  2023  14(1):4521  PubMed ID: 37607907   DOI: 10.1038/s41467-023-40243-8

21428  Honda K, Hitora Y, Tsukamoto S.  Akanthomins A-C, aphidicolin analogs from a fungus Akanthomyces sp., that inhibit cell cycle.  Phytochemistry  2023  216:113885  PubMed ID: 37806468   DOI: 10.1016/j.phytochem.2023.113885

20650  Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S.  Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.  Cells  2022  11(5)  PubMed ID: 35269503   DOI: 10.3390/cells11050881

13586  Hitora Y, Sejiyama A, Honda K, Ise Y, Losung F, Mangindaan REP, Tsukamoto S.  Fluorescent image-based high-content screening of extracts of natural resources for cell cycle inhibitors and identification of a new sesquiterpene quinone from the sponge, Dactylospongia metachromia  Bioorg Med Chem  2021  31:115968  PubMed ID: 33387695   DOI: 10.1016/j.bmc.2020.115968

13202  Iwata H, Shuto T, Kamei S, Omachi K, Moriuchi M, Omachi C, Toshito T, Hashimoto S, Nakajima K, Sugie C, Ogino H, Kai H, Shibamoto Y.  Combined effects of cisplatin and photon or proton irradiation in cultured cells: radiosensitization, patterns of cell death and cell cycle distribution.  J Radiat Res  2020    PubMed ID: 32880637   DOI: 10.1093/jrr/rraa065

13217  Yamanaka R, Shindo Y, Hotta K, Hiroi N, Oka K.  Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature.  Biochem Biophys Res Commun  2020    PubMed ID: 32928506   DOI: 10.1016/j.bbrc.2020.08.110

13333  Kim H, Watanabe S, Kitamatsu M, Watanabe K, Ohtsuki T.  Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate.  Sci Rep  2020    PubMed ID: 33154435   DOI: 10.1038/s41598-020-76100-7

14818  Shu S, Iimori M, Wakasa T, Ando K, Saeki H, Oda Y, Oki E, Maehara Y.  The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells  J Cell Sci  2019  132(24):jcs231530  PubMed ID: 31757888   DOI: 10.1242/jcs.231530

4211  Panagiotakopoulou M, Lendenmann T, Pramotton FM, Giampietro C, Stefopoulos G, Poulikakos D, Ferrari A.  Cell cycle-dependent force transmission in cancer cells.  Mol. Biol. Cell  2018    PubMed ID: 30113874   DOI: 10.1091/mbc.E17-12-0726

7021  Sasaki T, Sato Y, Higashiyama T, Sasaki N.  Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells.  Sci Rep  2017  7:11257  PubMed ID: 28900194   DOI: 10.1038/s41598-017-10843-8

10632  Tsuda Y, Iimori M, Nakashima Y, Nakanishi R, Ando K, Ohgaki K, Kitao H, Saeki H, Oki E, Maehara Y.  Mitotic slippage and the subsequent cell fates after inhibition of Aurora B during tubulin-binding agent-induced mitotic arrest.  Sci Rep  2017  7:16762  PubMed ID: 29196757   DOI: 10.1038/s41598-017-17002-z

10910    Intracellular calcium dynamics and expression of P2Y and IP3 receptors in a cycling G1-phase cell  Bioimages  2016  24 : 13-29    DOI: 10.11169/bioimages.24.13

13989  Asahina T, Kaida A, Goto T, Yoshimura R, Sasai K, Miura M.  Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading system (HDR-RALS)  Radiat Oncol  2016  11:99  PubMed ID: 27473168   DOI: 10.1186/s13014-016-0669-8

15170  Gao B, Konno T, Ishihara K.  Fabrication of a live cell-containing multilayered polymer hydrogel membrane with micrometer-scale thickness to evaluate pharmaceutical activity  J Biomater Sci Polym Ed  2015  26(18):1372-85  PubMed ID: 26374190   DOI: 10.1080/09205063.2015.1095025

15171  Botao Gao, Tomohiro Konno, Kazuhiko Ishihara  Building cell-containing multilayered phospholipid polymer hydrogels for controlling diffusion of bioactive reagent  RSC Adv  2015  5:44408-44415    DOI: 10.1039/C5RA05299H

16000  Murakami T, Aida Y.  Visualizing Vpr-induced G2 arrest and apoptosis  PLoS One  2014  9(1):e86840  PubMed ID: 24466265   DOI: 10.1371/journal.pone.0086840

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