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Cell No. : Cell Name
RCB2867 : HeLa/Fucci2  update : 2024/07/16
CommentA subline of the HeLa cell line expressing Fucci2, a cell cycle marker. Cell line name : HeLa/Fucci2 #3. Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositor
Terms and conditions1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2009
Another name HeLa.S-Fucci2
Original cell HeLa.S3
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci2
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U187
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 18

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7987  Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A.  Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.  BMC Cell Biol.  2011  12:2  PubMed ID: 21226962   DOI: 10.1186/1471-2121-12-2
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
21584  Kazama R, Sakai S.  Effect of cell adhesiveness of Cell Dome shell on enclosed HeLa cells.  J Biosci Bioeng  2024  137(4):313-320  PubMed ID: 38307767   DOI: 10.1016/j.jbiosc.2024.01.002
17619  Ebata H, Umeda K, Nishizawa K, Nagao W, Inokuchi S, Sugino Y, Miyamoto T, Mizuno D.  Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology  Biophys J  2023  ;S0006-3495(23)00241-2  PubMed ID: 37050875   DOI: 10.1016/j.bpj.2023.04.011
21373  Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.  TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.  Nat Commun  2023  14(1):4521  PubMed ID: 37607907   DOI: 10.1038/s41467-023-40243-8
21428  Honda K, Hitora Y, Tsukamoto S.  Akanthomins A-C, aphidicolin analogs from a fungus Akanthomyces sp., that inhibit cell cycle.  Phytochemistry  2023  216:113885  PubMed ID: 37806468   DOI: 10.1016/j.phytochem.2023.113885
20650  Mubarok W, Elvitigala KCML, Nakahata M, Kojima M, Sakai S.  Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels.  Cells  2022  11(5)  PubMed ID: 35269503   DOI: 10.3390/cells11050881
13586  Hitora Y, Sejiyama A, Honda K, Ise Y, Losung F, Mangindaan REP, Tsukamoto S.  Fluorescent image-based high-content screening of extracts of natural resources for cell cycle inhibitors and identification of a new sesquiterpene quinone from the sponge, Dactylospongia metachromia  Bioorg Med Chem  2021  31:115968  PubMed ID: 33387695   DOI: 10.1016/j.bmc.2020.115968
13202  Iwata H, Shuto T, Kamei S, Omachi K, Moriuchi M, Omachi C, Toshito T, Hashimoto S, Nakajima K, Sugie C, Ogino H, Kai H, Shibamoto Y.  Combined effects of cisplatin and photon or proton irradiation in cultured cells: radiosensitization, patterns of cell death and cell cycle distribution.  J Radiat Res  2020    PubMed ID: 32880637   DOI: 10.1093/jrr/rraa065
13217  Yamanaka R, Shindo Y, Hotta K, Hiroi N, Oka K.  Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature.  Biochem Biophys Res Commun  2020    PubMed ID: 32928506   DOI: 10.1016/j.bbrc.2020.08.110
13333  Kim H, Watanabe S, Kitamatsu M, Watanabe K, Ohtsuki T.  Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate.  Sci Rep  2020    PubMed ID: 33154435   DOI: 10.1038/s41598-020-76100-7
14818  Shu S, Iimori M, Wakasa T, Ando K, Saeki H, Oda Y, Oki E, Maehara Y.  The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells  J Cell Sci  2019  132(24):jcs231530  PubMed ID: 31757888   DOI: 10.1242/jcs.231530
4211  Panagiotakopoulou M, Lendenmann T, Pramotton FM, Giampietro C, Stefopoulos G, Poulikakos D, Ferrari A.  Cell cycle-dependent force transmission in cancer cells.  Mol. Biol. Cell  2018    PubMed ID: 30113874   DOI: 10.1091/mbc.E17-12-0726
7021  Sasaki T, Sato Y, Higashiyama T, Sasaki N.  Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells.  Sci Rep  2017  7:11257  PubMed ID: 28900194   DOI: 10.1038/s41598-017-10843-8
10632  Tsuda Y, Iimori M, Nakashima Y, Nakanishi R, Ando K, Ohgaki K, Kitao H, Saeki H, Oki E, Maehara Y.  Mitotic slippage and the subsequent cell fates after inhibition of Aurora B during tubulin-binding agent-induced mitotic arrest.  Sci Rep  2017  7:16762  PubMed ID: 29196757   DOI: 10.1038/s41598-017-17002-z
10910    Intracellular calcium dynamics and expression of P2Y and IP3 receptors in a cycling G1-phase cell  Bioimages  2016  24 : 13-29    DOI: 10.11169/bioimages.24.13
13989  Asahina T, Kaida A, Goto T, Yoshimura R, Sasai K, Miura M.  Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading system (HDR-RALS)  Radiat Oncol  2016  11:99  PubMed ID: 27473168   DOI: 10.1186/s13014-016-0669-8
15170  Gao B, Konno T, Ishihara K.  Fabrication of a live cell-containing multilayered polymer hydrogel membrane with micrometer-scale thickness to evaluate pharmaceutical activity  J Biomater Sci Polym Ed  2015  26(18):1372-85  PubMed ID: 26374190   DOI: 10.1080/09205063.2015.1095025
15171  Botao Gao, Tomohiro Konno, Kazuhiko Ishihara  Building cell-containing multilayered phospholipid polymer hydrogels for controlling diffusion of bioactive reagent  RSC Adv  2015  5:44408-44415    DOI: 10.1039/C5RA05299H
16000  Murakami T, Aida Y.  Visualizing Vpr-induced G2 arrest and apoptosis  PLoS One  2014  9(1):e86840  PubMed ID: 24466265   DOI: 10.1371/journal.pone.0086840

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