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Cell No. : Cell Name
RCB2864 : OLP6  update : 2023/10/16
CommentRat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2005;136:115-121) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hashimoto, Seiichi
Originator Matsushita, Tomoko
Year of deposit 2008
Animal _rat < Mammals
Strain name Wister (Transgenic rat)
Gender Unknown
Age at sampling 3 months
Tissue brain
Classification transformed
Recombinant recombinant
Exogene tsA58mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U466
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish type I collagen coated dish
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3633  Matsushita T, Amagai Y, Soga T, Terai K, Obinata M, Hashimoto S.  A novel oligodendrocyte cell line OLP6 shows the successive stages of oligodendrocyte development: late progenitor, immature and mature stages.  Neuroscience  2005  136(1):115-21  PubMed ID: 16181743   DOI: 10.1016/j.neuroscience.2005.07.011

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User's Publication
7025  Maekawa M, Watanabe A, Iwayama Y, Kimura T, Hamazaki K, Balan S, Ohba H, Hisano Y, Nozaki Y, Ohnishi T, Toyoshima M, Shimamoto C, Iwamoto K, Bundo M, Osumi N, Takahashi E, Takashima A, Yoshikawa T.  Polyunsaturated fatty acid deficiency during neurodevelopment in mice models the prodromal state of schizophrenia through epigenetic changes in nuclear receptor genes.  Transl Psychiatry  2017  7:e1229  PubMed ID: 28872641   DOI: 10.1038/tp.2017.182



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