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Cell No. : Cell Name
RCB2864 : OLP6  update : 2018/07/06
CommentRat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2005;136:115-121) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hashimoto, Seiichi
Originator Matsushita, Tomoko
Year of deposit 2008
Animal rat < Mammals
Strain name Wister (Transgenic rat)
Gender Unknown
Age at sampling 3 months
Tissue brain
Classification transformed
Recombinant recombinant
Exogene tsA58mutant
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF
Antibiotics Free
Passage method 0.25% trypsin
Coating dish type I collagen coated dish
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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3633  Matsushita T, Amagai Y, Soga T, Terai K, Obinata M, Hashimoto S.  A novel oligodendrocyte cell line OLP6 shows the successive stages of oligodendrocyte development: late progenitor, immature and mature stages.  Neuroscience  2005  136(1):115-21  PubMed ID: 16181743

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User's Publication
7025  Maekawa M, Watanabe A, Iwayama Y, Kimura T, Hamazaki K, Balan S, Ohba H, Hisano Y, Nozaki Y, Ohnishi T, Toyoshima M, Shimamoto C, Iwamoto K, Bundo M, Osumi N, Takahashi E, Takashima A, Yoshikawa T.  Polyunsaturated fatty acid deficiency during neurodevelopment in mice models the prodromal state of schizophrenia through epigenetic changes in nuclear receptor genes.  Transl Psychiatry  2017  7:e1229  PubMed ID: 28872641

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