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Cell No. : Cell Name
RCB2863 : N14.5  update : 2023/10/16
CommentRat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2006;140:849-856) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hashimoto, Seiichi
Originator Matsushita, Tomoko
Year of deposit 2008
Animal _rat < Mammals
Strain name Wister (Transgenic rat)
Gender Unknown
Age at sampling 3 months
Tissue brain
Classification transformed
Recombinant recombinant
Exogene tsA58mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U465
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish type I collagen coated dish
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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3632  Matsushita T, Amagai Y, Terai K, Kojima T, Obinata M, Hashimoto S.  A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus.  Neuroscience  2006  140(3):849-56  PubMed ID: 16616428   DOI: 10.1016/j.neuroscience.2006.02.059

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User's Publication
9641  Nagamoto S, Iijima N, Ishii H, Takumi K, Higo S, Aikawa S, Anzai M, Matsuo I, Nakagawa S, Takashima N, Shigeyoshi Y, Sakamoto A, Ozawa H.  Establishment of an in vitro cell line experimental system for the study of inhalational anesthetic mechanisms.  Neurosci. Lett.  2016  620:163-8  PubMed ID: 27057734   DOI: 10.1016/j.neulet.2016.04.005

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