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Cell No. : Cell Name
RCB2863 : N14.5 update : 2018/07/06 |
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| Comment | Rat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus | |||
| Comment from the depositor | ||||
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2006;140:849-856) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
| Basic information | Depositor | Hashimoto, Seiichi | ||
| Originator | Matsushita, Tomoko | |||
| Year of deposit | 2008 | |||
| Animal | rat < Mammals | |||
| Strain name | Wister (Transgenic rat) | |||
| Gender | Unknown | |||
| Age at sampling | 3 months | |||
| Tissue | brain | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | tsA58mutant | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Medium and additives | Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF | |||
| Antibiotics | Free | Passage method | 0.25% trypsin | |
| Coating dish | type I collagen coated dish | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
| Temperature | 33 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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