Cell No. : Cell Name
RCB2863 : N14.5 update : 2018/07/06 |
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Comment | Rat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus | |||
Comment from the depositor | ||||
Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2006;140:849-856) designated by the DEPOSITOR is required. | |||
Remarks | ||||
Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
Basic information | Depositor | Hashimoto, Seiichi | ||
Originator | Matsushita, Tomoko | |||
Year of deposit | 2008 | |||
Animal | rat < Mammals | |||
Strain name | Wister (Transgenic rat) | |||
Gender | Unknown | |||
Age at sampling | 3 months | |||
Tissue | brain | |||
Classification | transformed | |||
Recombinant | recombinant | |||
Exogene | tsA58mutant | Lifespan | infinite | |
Morphology | fibroblast-like | |||
Medium | Medium List | Culture type | Adherent cells | |
Medium and additives | Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF | |||
Antibiotics | Free | Passage method | 0.25% trypsin | |
Coating dish | type I collagen coated dish | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
Temperature | 33 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
Images | ||||
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Reference information | Reference | 1 | ||
User's Publication | 1 | |||