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Cell No. : Cell Name
RCB2863 : N14.5 update : 2021/05/20 |
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| Comment | Rat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus | |||
| Comment from the depositor | ||||
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2006;140:849-856) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Hashimoto, Seiichi | ||
| Originator | Matsushita, Tomoko | |||
| Year of deposit | 2008 | |||
| Animal | rat < Mammals | |||
| Strain name | Wister (Transgenic rat) | |||
| Gender | Unknown | |||
| Age at sampling | 3 months | |||
| Tissue | brain | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | tsA58mutant | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_U465 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Medium and additives | Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | |
| Coating dish | type I collagen coated dish | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
| Temperature | 33 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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