Cell No. : Cell Name RCB2863 : N14.5  update : 2023/10/16
Comment			Rat neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neuroscience 2006;140:849-856) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hashimoto, Seiichi
		Originator	Matsushita, Tomoko
		Year of deposit	2008
		Animal	_rat < Mammals
		Strain name	Wister (Transgenic rat)
		Gender	Unknown
		Age at sampling	3 months
		Tissue	brain
		Classification	transformed
		Recombinant	recombinant
		Exogene	tsA58mutant
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_U465
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		Neurobasal Medium (NBM) + 0.5mM L-Glutamine + 2% B27 + 5% FBS + 20ng/ml EGF + 20ng/ml basic FGF
		Antibiotics		Free
		Passage method		0.25% Trypsin
		Coating dish		type I collagen coated dish
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		33 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	1

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Reference

3632  Matsushita T, Amagai Y, Terai K, Kojima T, Obinata M, Hashimoto S.  A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus.  Neuroscience  2006  140(3):849-56  PubMed ID: 16616428   DOI: 10.1016/j.neuroscience.2006.02.059

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User's Publication

9641  Nagamoto S, Iijima N, Ishii H, Takumi K, Higo S, Aikawa S, Anzai M, Matsuo I, Nakagawa S, Takashima N, Shigeyoshi Y, Sakamoto A, Ozawa H.  Establishment of an in vitro cell line experimental system for the study of inhalational anesthetic mechanisms.  Neurosci. Lett.  2016  620:163-8  PubMed ID: 27057734   DOI: 10.1016/j.neulet.2016.04.005