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Cell No. : Cell Name
RCB2862 : SLC  update : 2024/01/17
CommentRat cell line derived from lung cancer. TKG0135 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Another name Sato lung cancer
Original cell TKG0135
Animal _rat < Mammals
Strain name Donryu
Tissue lung
Disease name lung cancer
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0135)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5365
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, PepB
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
5367  Saijo N, Niitani H, Irimajiri N, Chikama M.  Effect of 1(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) on Sato lung carcinoma (SLC). Preliminary result of immunochemotherapy for SLC by combination of ACNU and Propionibacterium acnes.  Oncology  1979  36(1):7-10  PubMed ID: 450390   DOI: 10.1159/000225309
5368  Chikama M, Saijo N, Irimajiri N, Niitani H.  Effect of BCG on cytostatic activity of peritoneal macrophages from normal and tumor-bearing rats.  Gan  1979  70(2):229-33  PubMed ID: 467886  
5366  Saijo N, Niitani H, Chikama M, Taniguchi T, Kimura K.  Effect of Propionibacterium acnes or BCG on enzyme activities in spleen lymphocytes of Donryu strain rats.  Gan  1978  69(3):345-52  PubMed ID: 149684  

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User's Publication
21449  Kasumi E, Chiba M, Kuzumaki Y, Kuzuoka H, Sato N, Takahashi B.  Development and Characterization of a Cancer Cachexia Rat Model Transplanted with Cells of the Rat Lung Adenocarcinoma Cell Line Sato Lung Cancer (SLC).  Biomedicines  2023  11(10)  PubMed ID: 37893197   DOI: 10.3390/biomedicines11102824



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