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Cell No. : Cell Name
RCB2854 : C6  update : 2021/02/26
CommentRat cell line derived from glial cell tumor induced by N-nitrosomethylurea. TKG0589 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0589
Animal _rat < Mammals
Tissue brain
Disease name glial cell tumor
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0589)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, PepB
deposit info
lot info
Reference information Reference 6
User's Publication 14

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5354  Zagyansky Y, Benda P, Bisconte JC.  Restricted lateral diffusion of concanavalin A receptors of different malignant cells of the nervous system.  FEBS Lett  1977  77(2):206-8  PubMed ID: 862919
5353  Rieger F, Faivre-Bauman A, Benda P, Vigny M.  Molecular forms of acetylcholinesterase: their de novo synthesis in mouse neuroblastoma cells.  J Neurochem  1976  27(5):1059-63  PubMed ID: 12170588
5352  Premont J, Benda P, Jard S.  [3H] norepinephrine binding by rat glial cells in culture. Lack of correlation between binding and adenylate cyclase activation.  Biochim Biophys Acta  1975  381(2):368-76  PubMed ID: 163103
5351  Penit J, Jard S, Benda P.  Probenecide sensitive 3'-5'-cyclic AMP secretion by isoproterenol stimulated glial cells in culture.  FEBS Lett  1974  41(1):156-60  PubMed ID: 4369045
5350  Jard S, Premont J, Benda P.  Adenylate cyclase, phosphodiesterases and protein kinase of rat glial cells in culture.  FEBS Lett  1972  26(1):344-8  PubMed ID: 4344295
645  Benda P, Lightbody J, Sato G, Levine L, Sweet W.  Differentiated rat glial cell strain in tissue culture.  Science  1968  161(3839):370-1  PubMed ID: 4873531

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User's Publication
6917  Watabe T et al  Theranostics targeting fibroblast activation protein in the tumor stroma: 64Cu and 225Ac labelled FAPI-04 in pancreatic cancer xenograft mouse models.  J Nucl Med  2020  61(4):563-569  PubMed ID: 31586001
12991  Watabe T, Kaneda-Nakashima K, Shirakami Y, Liu Y, Ooe K, Teramoto T, Toyoshima A, Shimosegawa E, Nakano T, Kanai Y, Shinohara A, Hatazawa J.  Targeted alpha therapy using astatine (211At)-labeled phenylalanine: A preclinical study in glioma bearing mice.  Oncotarget  2020    PubMed ID: 32341757
13601  Hirai N, Watabe T, Nagamori S, Wiriyasermkul P, Tanaka Y, Romanov V, Naka S, Kanai Y, Liu Y, Tani N, Sakai T, Tatsumi M, Shimosegawa E, Kanai Y, Hatazawa J.  Evaluation of D-isomer of 18 F-FBPA for oncology PET focusing on the differentiation of glioma and inflammation  Asia Ocean J Nucl Med Biol  2020  8(2):102-108.  PubMed ID: 32714997
5357  Takahashi D, Moriyama J, Nakamura T, Miki E, Takahashi E, Sato A, Akaike T, Itto-Nakama K, Arimoto H.  AUTACs: Cargo-Specific Degraders Using Selective Autophagy.  Mol Cell  2019  76(5):797-810  PubMed ID: 31606272
11209  Yamada D, Fujikawa K, Kawabe K, Furuta T, Nakada M, Takarada T.  RUNX2 Promotes Malignant Progression in Glioma.  Neurochem. Res.  2018    PubMed ID: 30203400
9806  Watabe T, Ikeda H, Nagamori S, Wiriyasermkul P, Tanaka Y, Naka S, Kanai Y, Hagiwara K, Aoki M, Shimosegawa E, Kanai Y, Hatazawa J.  18F-FBPA as a tumor-specific probe of L-type amino acid transporter 1 (LAT1): a comparison study with 18F-FDG and 11C-Methionine PET.  Eur. J. Nucl. Med. Mol. Imaging  2017  44:321-331  PubMed ID: 27550420
10133  Watabe T, Kanai Y, Ikeda H, Horitsugi G, Matsunaga K, Kato H, Isohashi K, Abe K, Shimosegawa E, Hatazawa J.  Quantitative evaluation of oxygen metabolism in the intratumoral hypoxia: 18F-fluoromisonidazole and 15O-labelled gases inhalation PET.  EJNMMI Res  2017  7:16  PubMed ID: 28210996
10283  Watabe T, Hanaoka K, Naka S, Kanai Y, Ikeda H, Aoki M, Shimosegawa E, Kirihata M, Hatazawa J.  Practical calculation method to estimate the absolute boron concentration in tissues using 18F-FBPA PET.  Ann Nucl Med  2017  31:481-485  PubMed ID: 28439784
9917  Podyma-Inoue KA, Moriwaki T, Rajapakshe AR, Terasawa K, Hara-Yokoyama M.  Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells.  Cancer Genomics Proteomics  2016  13:443-452  PubMed ID: 27807067
7345  Yamaguchi A, Hanaoka H, Fujisawa Y, Zhao S, Suzue K, Morita A, Tominaga H, Higuchi T, Hisaeda H, Tsushima Y, Kuge Y, Iida Y.  Differentiation of malignant tumours from granulomas by using dynamic [(18)F]-fluoro-L-α-methyltyrosine positron emission tomography.  EJNMMI Res  2015  5:29  PubMed ID: 25977883
9473  Koichi Fujikawa, Ryo Fukumori, Saki Nakamura, Takaya Kutsukake, Takeshi Takarada, Yukio Yoneda  Potential Interactions of Calcium-Sensitive Reagents With Zinc Ion in Different Cultured Cells  PLoS One  2015  10(5):e0127421  PubMed ID: 26010609
5999  Zhao S, Liu H, Liu Y, Wu J, Wang C, Hou X, Chen X, Yang G, Zhao L, Che H, Bi Y, Wang H, Peng F, Ai J.  miR-143 inhibits glycolysis and depletes stemness of glioblastoma stem-like cells.  Cancer Lett  2013  333(2):253-60  PubMed ID: 23376635
7999  Ogura M, Takarada T, Nakamichi N, Kawagoe H, Sako A, Nakazato R, Yoneda Y.  Exacerbated vulnerability to oxidative stress in astrocytic C6 glioma cells with stable overexpression of the glutamine transporter slc38a1.  Neurochem. Int.  2011  58:504-11  PubMed ID: 21219957
9527  Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami, Masataka Yoshino  Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells  Neurochem Res . 2004 Apr;29(4):687-93  2004  29(4):687-93  PubMed ID: 15098930

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