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Cell No. : Cell Name
RCB2853 : WEHI-3B  update : 2023/10/16
CommentMouse cell line derived from myelomonocytic leukemia cells. BALB/c strain. TKG0584 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Warner, N.L.
Year of deposit 2008
Original cell TKG0584
Animal _mouse < Mammals
Strain name BALB/c
Tissue myelomonocyte
Disease name myelomonocytic leukemia cell lin
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0584)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2239
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 4

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5288  Shimizu T, Izumi T, Seyama Y, Tadokoro K, Rådmark O, Samuelsson B.  Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase.  Proc Natl Acad Sci U S A  1986  83(12):4175-9  PubMed ID: 3012557   DOI: 10.1073/pnas.83.12.4175
5287  McKean DJ, Infante AJ, Nilson A, Kimoto M, Fathman CG, Walker E, Warner N.  Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.  J Exp Med  1981  154(5):1419-31  PubMed ID: 6170720   DOI: 10.1084/jem.154.5.1419
1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922  
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528
5289  Metcalf D, Moore MA, Warner NL.  Colony formation in vitro by myelomonocytic leukemic cells.  J Natl Cancer Inst  1969  43(4):983-1001  PubMed ID: 5259326  

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User's Publication
21565  Yamaguchi M, Sera Y, Toga-Yamaguchi H, Kanegane H, Iguchi Y, Fujimura K.  Knockdown of the Shwachman-Diamond syndrome gene, SBDS, induces galectin-1 expression and impairs cell growth.  Int J Hematol  2024    PubMed ID: 38240987   DOI: 10.1007/s12185-024-03709-z
12115  Hirai N, Sasaki T, Okumura S, Minami Y, Chiba S, Ohsaki Y.  Monomerization of ALK Fusion Proteins as a Therapeutic Strategy in ALK-Rearranged Non-small Cell Lung Cancers.  Front Oncol  2020    PubMed ID: 32300555   DOI: 10.3389/fonc.2020.00419
9506  Nakaoku T, Kohno T, Araki M, Niho S, Chauhan R, Knowles PP, Tsuchihara K, Matsumoto S, Shimada Y, Mimaki S, Ishii G, Ichikawa H, Nagatoishi S, Tsumoto K, Okuno Y, Yoh K, McDonald NQ, Goto K.  A secondary RET mutation in the activation loop conferring resistance to vandetanib.  Nat Commun  2018  9:625  PubMed ID: 29434222   DOI: 10.1038/s41467-018-02994-7
14651  Shimada Y, Kohno T, Ueno H, Ino Y, Hayashi H, Nakaoku T, Sakamoto Y, Kondo S, Morizane C, Shimada K, Okusaka T, Hiraoka N.  An Oncogenic ALK Fusion and an RRAS Mutation in KRAS Mutation-Negative Pancreatic Ductal Adenocarcinoma  Oncologist  2017  22(2):158-164  PubMed ID: 28167572   DOI: 10.1634/theoncologist.2016-0194

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