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Cell No. : Cell Name
RCB2852 : TSA8  update : 2022/01/07
CommentMouse cell line derived from erythroleukemia cells transformed by Friend leukemia virus. TKG0370 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Tak Mak.
Year of deposit 2008
Original cell TKG0370
Animal mouse < Mammals
Tissue spleen
Disease name erythroleukemia cell line
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0370)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_A799
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (4mM L-Glutamine) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
5286  Noguchi T, Fukumoto H, Mishina Y, Obinata M.  Factors controlling induction of commitment of murine erythroleukemia (TSA8) cells to CFU-E (colony forming unit-erythroid).  Development  1987  101(1):169-74  PubMed ID: 2835222  
5285  Mishina Y, Obinata M.  Induction of commitment of murine erythroleukemia cells (TSA8) to CFU-E with DMSO.  Exp Cell Res  1986  162(2):319-25  PubMed ID: 3455915   DOI: 10.1016/0014-4827(86)90337-x
5284  Shibuya T, Mak TW.  Isolation and induction of erythroleukemic cell lines with properties of erythroid progenitor burst-forming cell (BFU-E) and erythroid precursor cell (CFU-E).  Proc Natl Acad Sci U S A  1983  80(12):3721-5  PubMed ID: 6574510   DOI: 10.1073/pnas.80.12.3721

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