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Cell No. : Cell Name
RCB2848 : OTT6050(B)  update : 2021/05/20
CommentMouse cell line derived from testicular teratoma. This cell line was established by inoculation of tibia (6 days old) to A/He x 129/sv mouse testis. Primary tumor was developed in testis. TKG0330 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0330
Animal mouse < Mammals
Genus Mus
Species musculus
Tissue testis
Disease name testicular teratoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0330)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4U64
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5283  Stevens LC.  The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos.  Dev Biol  1970  21(3):364-82  PubMed ID: 5436899

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