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Cell No. : Cell Name
RCB2846 : LAG-MUC1  update : 2021/05/20
CommentA subline of LAG (RCB2758) expressing MUC1. 8-Ag resistant. TKG0466 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kudo T.
Year of deposit 2008
Original cell TKG0466
Animal mouse < Mammals
Strain name C3H
Tissue connective tissue
Classification transformed
Recombinant recombinant
Exogene MUC1 plasmid (APR-MUC1)
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0466)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B951
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 5
User's Publication 0


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Reference
5262  Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.  Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.  J Cell Sci  1991  100 ( Pt 4):841-9  PubMed ID: 1814933
5260  Dei T, Masuko K, Ishii H, Makita T, Tachibana T.  Novel antigen expression on syngeneic somatic cell hybrids of murine spontaneous mammary tumor cells.  J Natl Cancer Inst  1986  77(2):511-9  PubMed ID: 3461212
5259  Ishikawa S, Tachibana T.  Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells.  J Immunol  1983  131(4):2059-63  PubMed ID: 6194225
5261  Dei T, Tachibana T.  Induced resistance in normal mice and growth enhancement in tumor-bearing mice by inoculation with syngeneic hybrid cells.  J Natl Cancer Inst  1980  65(4):739-49  PubMed ID: 6448309
5258  Murayama F, Okada Y.  Efficiency of hybrid cell formation from heterokaryons fused by HVI.  Biken J  1970  13(1):1-9  PubMed ID: 4316425

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