Cell No. : Cell Name
RCB2846 : LAG-MUC1
update : 2023/10/16
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| Comment | A subline of LAG (RCB2758) expressing MUC1. 8-Ag resistant. TKG0466 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fukuda, Hiroshi
|
| Originator |
Kudo T.
|
| Year of deposit |
2008
|
| Original cell |
TKG0466
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Tissue |
connective tissue
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
MUC1 plasmid (APR-MUC1)
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0466）
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_B951
|
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
|
Adherent cells
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
5262
Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.
Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.
J Cell Sci
1991
100 ( Pt 4):841-9
PubMed ID: 1814933
DOI: 10.1242/jcs.100.4.841
|
|
5260
Dei T, Masuko K, Ishii H, Makita T, Tachibana T.
Novel antigen expression on syngeneic somatic cell hybrids of murine spontaneous mammary tumor cells.
J Natl Cancer Inst
1986
77(2):511-9
PubMed ID: 3461212
|
|
5259
Ishikawa S, Tachibana T.
Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells.
J Immunol
1983
131(4):2059-63
PubMed ID: 6194225
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5261
Dei T, Tachibana T.
Induced resistance in normal mice and growth enhancement in tumor-bearing mice by inoculation with syngeneic hybrid cells.
J Natl Cancer Inst
1980
65(4):739-49
PubMed ID: 6448309
DOI: 10.1093/jnci/65.4.739
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|
5258
Murayama F, Okada Y.
Efficiency of hybrid cell formation from heterokaryons fused by HVI.
Biken J
1970
13(1):1-9
PubMed ID: 4316425
|
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