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Cell No. : Cell Name
RCB2845 : L1210-5FU-R-TC  update : 2024/01/17
CommentMouse cell line derived from leukemia. Lymphoblastoid cells. DBA/2 strain. TKG0409 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Saeki, H.
Year of deposit 2008
Original cell TKG0409
Animal _mouse < Mammals
Strain name DBA/2
Tissue spleen, lymphnode, leukemia
Disease name leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0409)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_C513
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


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