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Cell No. : Cell Name
RCB2843 : 43L  update : 2021/05/20
CommentSomatic tumor hybrid (fusion cell between C3H mammary tumor FM3A#2 and 8-Ag resistant L cell). This hybrid tumor cells induce resistance to MM Ag positive tumor cells. TKG0381 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Dei, T. & Tachibana, T.
Year of deposit 2008
Original cell TKG0381
Animal mouse x mouse < Hybrid
Tissue hybrid cell , FM3A#2 x L cell (8-Ag resistant)
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0381)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3356
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 0

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5282  Kambe M, Rou K, Tachibana T.  Differences in immune responses to tumor induced in syngeneic hosts by injection of hybrid and parental tumor cells.  Tohoku J Exp Med  1994  174(1):71-83  PubMed ID: 7863505
5281  Ishikawa S, Tachibana T.  Killer-helper effect of L cells in the generation of anti-MM cytotoxic T lymphocytes: replacement by beta-interferon and mechanisms of action.  Jpn J Cancer Res  1986  77(9):931-40  PubMed ID: 2945807
5259  Ishikawa S, Tachibana T.  Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells.  J Immunol  1983  131(4):2059-63  PubMed ID: 6194225

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