Cell No. : Cell Name RCB2843 : 43L  update : 2023/10/16
Comment			Somatic tumor hybrid (fusion cell between C3H mammary tumor FM3A#2 and 8-Ag resistant L cell). This hybrid tumor cells induce resistance to MM Ag positive tumor cells. TKG0381 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Dei, T. & Tachibana, T.
		Year of deposit	2008
		Original cell	TKG0381
		Animal	_mouse x mouse < Hybrid
		Tissue	hybrid cell , FM3A#2 x L cell (8-Ag resistant)
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0381）
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_3356
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	0

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Reference

5282  Kambe M, Rou K, Tachibana T.  Differences in immune responses to tumor induced in syngeneic hosts by injection of hybrid and parental tumor cells.  Tohoku J Exp Med  1994  174(1):71-83  PubMed ID: 7863505   DOI: 10.1620/tjem.174.71

5281  Ishikawa S, Tachibana T.  Killer-helper effect of L cells in the generation of anti-MM cytotoxic T lymphocytes: replacement by beta-interferon and mechanisms of action.  Jpn J Cancer Res  1986  77(9):931-40  PubMed ID: 2945807

5259  Ishikawa S, Tachibana T.  Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells.  J Immunol  1983  131(4):2059-63  PubMed ID: 6194225

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