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Cell No. : Cell Name
RCB2834 : L12.9-2(1)  update : 2023/10/16
CommentHuman immortalized cell line derived from MRC5 cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Takano, Toshiya
Year of deposit 2008
Original cell MRC-5
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling fetus
Tissue lung
Classification transformed
Recombinant recombinant
Exogene SV40 T antigen
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U651
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 4
User's Publication 0

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900  H Ikeshima, S Imai, K Shimoda, J Hata, T Takano  Expression of a MADS box gene, MEF2D, in neurons of the mouse central nervous system: implication of its binary function in myogenic and neurogenic cell lineages  Neurosci Lett  1995  200(2):117-20  PubMed ID: 8614558   DOI: 10.1016/0304-3940(95)12092-i
899  S Imai, T Fujino, S Nishibayashi, T Manabe, T Takano  Immortalization-susceptible elements and their binding factors mediate rejuvenation of regulation of the type I collagenase gene in simian virus 40 large T antigen-transformed immortal human fibroblasts  Mol Cell Biol  1994  14(11):7182-94  PubMed ID: 7935433   DOI: 10.1128/mcb.14.11.7182
898  S Imai, F Saito, T Ikeuchi, K Segawa, T Takano  Escape from in vitro aging in SV40 large T antigen-transformed human diploid cells: a key event responsible for immortalization occurs during crisis  Mech Ageing Dev  1993  69(1-2):149-58  PubMed ID: 8397328   DOI: 10.1016/0047-6374(93)90079-7
897  S Imai , T Takano  Loss of collagenase gene expression in immortalized clones of SV40 T antigen-transformed human diploid fibroblasts  Biochem Biophys Res Commun  1992  189(1):148-53  PubMed ID: 1280416   DOI: 10.1016/0006-291x(92)91537-z

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