Cell No. : Cell Name
RCB2834 : L12.9-2(1)
update : 2023/10/16
|
| Comment | Human immortalized cell line derived from MRC5 cells. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Takano, Toshiya
|
| Year of deposit |
2008
|
| Original cell |
MRC-5
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
fetus
|
| Tissue |
lung
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
SV40 T antigen
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_U651
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium : 2 x FM1 = 1 : 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
900
H Ikeshima, S Imai, K Shimoda, J Hata, T Takano
Expression of a MADS box gene, MEF2D, in neurons of the mouse central nervous system: implication of its binary function in myogenic and neurogenic cell lineages
Neurosci Lett
1995
200(2):117-20
PubMed ID: 8614558
DOI: 10.1016/0304-3940(95)12092-i
|
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899
S Imai, T Fujino, S Nishibayashi, T Manabe, T Takano
Immortalization-susceptible elements and their binding factors mediate rejuvenation of regulation of the type I collagenase gene in simian virus 40 large T antigen-transformed immortal human fibroblasts
Mol Cell Biol
1994
14(11):7182-94
PubMed ID: 7935433
DOI: 10.1128/mcb.14.11.7182
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898
S Imai, F Saito, T Ikeuchi, K Segawa, T Takano
Escape from in vitro aging in SV40 large T antigen-transformed human diploid cells: a key event responsible for immortalization occurs during crisis
Mech Ageing Dev
1993
69(1-2):149-58
PubMed ID: 8397328
DOI: 10.1016/0047-6374(93)90079-7
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897
S Imai , T Takano
Loss of collagenase gene expression in immortalized clones of SV40 T antigen-transformed human diploid fibroblasts
Biochem Biophys Res Commun
1992
189(1):148-53
PubMed ID: 1280416
DOI: 10.1016/0006-291x(92)91537-z
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