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Cell No. : Cell Name
RCB2831 : DEOC-1  update : 2022/09/13
CommentHuman cell line derived from malignant melanoma.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Hum Cell 2007;20:23-38) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamada, Takashi
Originator Yamada, Takashi
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 59 years
Tissue right heel
Disease name malignant melanoma
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
Year of origin 1999
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4161
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3630  Maeshima S, Yamada T, Kiyokane K, Mori H.  Establishment and characterization of a cell line (DEOC-1) originating from a human malignant melanoma of the skin.  Hum Cell  2007  20(2):23-38  PubMed ID: 17547717   DOI: 10.1111/j.1749-0774.2007.00028.x

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User's Publication
4187  Fujiwara S, Nagai H, Jimbo H, Jimbo N, Tanaka T, Inoie M, Nishigori C.  Gene Expression and Methylation Analysis in Melanomas and Melanocytes From the Same Patient: Loss of NPM2 Expression Is a Potential Immunohistochemical Marker for Melanoma.  Front Oncol  2019  8:675  PubMed ID: 30719424   DOI: 10.3389/fonc.2018.00675



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