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Cell No. : Cell Name
RCB2828 : N6803(>500)  update : 2024/07/09
CommentHuman B-lymphoblastoid cell lines transformed by Epstein-Barr virus. RCB2827, RCB2828 were derived from the same patient.
Comment from the depositor
Terms and conditions
Remarks
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SUGIMOTO, Masanobu
Originator SUGIMOTO, Masanobu
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 50 yesrs
Tissue blood
Classification transformed
Lifespan infinite
Morphology lymphoblast-like
Contact inhibition No
Tumorigenesis Yes
Cellosaurus(Expasy) CVCL_A6SD
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + FBS (10%) , pH 6.0 RPMI1640 + 10% FBS
Antibiotics Penicillin, Streptomycin Free
Passage method dilution
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 7.5 % 7.5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
STR(human) OK
Reference information Reference 11
User's Publication 0


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Reference
874  Futami K, Ishikawa Y, Goto M, Furuichi Y, Sugimoto M.  Role of Werner syndrome gene product helicase in carcinogenesis and in resistance to genotoxins by cancer cells  Cancer Sci  2008  99(5):843-8  PubMed ID: 18312465   DOI: 10.1111/j.1349-7006.2008.00778.x
7401  Sugimoto M, Tahara H, Okubo M, Kobayashi T, Goto M, Ide T, Furuichi Y.  WRN gene and other genetic factors affecting immortalization of human B-lymphoblastoid cell lines transformed by Epstein-Barr virus.  Cancer Genet Cytogenet  2004  152(2):95-100  PubMed ID: 15262425   DOI: 10.1016/j.cancergencyto.2003.11.005
7405  Sugimoto M, Tahara H, Ide T, Furuichi Y.  Steps involved in immortalization and tumorigenesis in human B-lymphoblastoid cell lines transformed by Epstein-Barr virus.  Cancer Res  2004  64(10):3361-4  PubMed ID: 15150084   DOI: 10.1158/0008-5472.CAN-04-0079
7351  Takahashi T, Kawabe T, Okazaki Y, Itoh C, Noda K, Tajima M, Satoh M, Goto M, Mitsui Y, Tahara H, Ide T, Furuichi Y, Sugimoto M.  In vitro establishment of tumorigenic human B-lymphoblastoid cell lines transformed by Epstein-Barr virus.  DNA Cell Biol  2003  22(11):727-35  PubMed ID: 14659045   DOI: 10.1089/104454903770946700
877  Toda T, Sugimoto M.  Proteome analysis of Epstein-Barr virus-transformed B-lymphoblasts and the proteome database  J Chromatogr B Analyt Technol Biomed Life Sci  2003  787(1):197-206  PubMed ID: 12659741   DOI: 10.1016/s1570-0232(02)00495-6
7317  Okubo M, Tsurukubo Y, Higaki T, Kawabe T, Goto M, Murase T, Ide T, Furuichi Y, Sugimoto M.  Clonal chromosomal aberrations accompanied by strong telomerase activity in immortalization of human B-lymphoblastoid cell lines transformed by Epstein-Barr virus.  Cancer Genet Cytogenet  2001  129(1):30-4  PubMed ID: 11520562   DOI: 10.1016/s0165-4608(01)00420-4
7407  Sugimoto M, Furuichi Y, Ide T, Goto M.  Incorrect us of "immortalization" for B-lymphoblastoid cell lines transformed by Epstein-Barr virus.  J Virol  1999  73(11):9690-1  PubMed ID: 10577062   DOI: 10.1128/JVI.73.11.9690-9691.1999
222  Okada M, Goto M, Furuichi Y, Sugimoto M.  Differential effects of cytotoxic drugs on mortal and immortalized B-lymphoblastoid cell lines from normal and Werner's syndrome patients  Biol Pharm Bull  1998  21(3):235-9  PubMed ID: 9556152   DOI: 10.1248/bpb.21.235
221  Kataoka H, Tahara H, Watanabe T, Sugawara M, Ide T, Goto M, Furuichi Y, Sugimoto M.  Immortalization of immunologically committed Epstein-Barr virus-transformed human B-lymphoblastoid cell lines accompanied by a strong telomerase activity  Differentiation   1997  62(4):203-11  PubMed ID: 9503605   DOI: 10.1046/j.1432-0436.1998.6240203.x
7082  Tahara H, Tokutake Y, Maeda S, Kataoka H, Watanabe T, Satoh M, Matsumoto T, Sugawara M, Ide T, Goto M, Furuichi Y, Sugimoto M.  Abnormal telomere dynamics of B-lymphoblastoid cell strains from Werner's syndrome patients transformed by Epstein-Barr virus.  Oncogene  1997  15(16):1911-20  PubMed ID: 9365237   DOI: 10.1038/sj.onc.1201377
7412  Counter CM, Botelho FM, Wang P, Harley CB, Bacchetti S.  Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes.  J Virol  1994  68(5):3410-4  PubMed ID: 8151802   DOI: 10.1128/JVI.68.5.3410-3414.1994

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