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Cell No. : Cell Name
RCB2818 : B1203L  update : 2021/05/20
CommentHuman cell line derived from lung adenocarcinoma. HLA A2402/2402, B5201/5401, C0102/1202.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Int J Cancer 2006;118:1992-1997 ) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yasumoto, Kosei
Originator Yasumoto, Kosei
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 52 years
Tissue lung
Disease name adenocarcinoma
Metastastatic ability Yes
Classification cancer
Year of origin 1997
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8658
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 4
User's Publication 0

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8991  Manabu Yasuda, Takeshi Hanagiri, Yoshiki Shigematsu, Takamitsu Onitsuka, Kouji Kuroda, Tetsurou Baba, Makiko Mizukami, Yoshinobu Ichiki, Hidetaka Uramoto, Mitsuhiro Takenoyama, Kosei Yasumoto  Identification of a Tumour Associated Antigen in Lung Cancer Patients With Asbestos Exposure  Anticancer Res  2010  30 (7), 2631-9  PubMed ID: 20682992  
8995  Takashi Fukuyama, Yoshinobu Ichiki, Sousuke Yamada, Yoshiki Shigematsu, Tetsuro Baba, Yoshika Nagata, Makiko Mizukami, Masakazu Sugaya, Mitsuhiro Takenoyama, Takeshi Hanagiri, Kenji Sugio, Kosei Yasumoto  Cytokine Production of Lung Cancer Cell Lines: Correlation Between Their Production and the Inflammatory/Immunological Responses Both in Vivo and in Vitro  Cancer Sci  2007  98 (7), 1048-54  PubMed ID: 17511773   DOI: 10.1111/j.1349-7006.2007.00507.x
3627  Takenoyama M, Baurain JF, Yasuda M, So T, Sugaya M, Hanagiri T, Sugio K, Yasumoto K, Boon T, Coulie PG.  A point mutation in the NFYC gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human squamous cell lung carcinoma.  Int J Cancer  2006  118(8):1992-7  PubMed ID: 16287085   DOI: 10.1002/ijc.21594
3636  Takenoyama M, Yoshino I, Eifuku R, So T, Imahayashi S, Sugaya M, Yasuda M, Inoue M, Ichiyoshi Y, Osaki T, Nagashima A, Nomoto K, Yasumoto K.  Successful induction of tumor-specific cytotoxic T lymphocytes from patients with non-small cell lung cancer using CD80-transfected autologous tumor cells.  Jpn J Cancer Res  2001  92(3):309-15  PubMed ID: 11267941   DOI: 10.1111/j.1349-7006.2001.tb01096.x

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