Cell No. : Cell Name RCB2817 : A529L  update : 2024/04/04
Comment			Human cell line derived from lung adenosquamous cell carcinoma. HLA A2402/, B5201/, Cw01201/.
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Jpn J Clin Oncol 2001;31:311-317) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Yasumoto, Kosei
		Originator	Yasumoto, Kosei
		Year of deposit	2008
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	72 years
		Tissue	lung
		Disease name	adenosquamous cell carcinoma
		Metastatic ability	Yes
		Classification	cancer
		Year of origin	1996
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_8655
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/2 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	6
		User's Publication	2

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Reference

8991  Manabu Yasuda, Takeshi Hanagiri, Yoshiki Shigematsu, Takamitsu Onitsuka, Kouji Kuroda, Tetsurou Baba, Makiko Mizukami, Yoshinobu Ichiki, Hidetaka Uramoto, Mitsuhiro Takenoyama, Kosei Yasumoto  Identification of a Tumour Associated Antigen in Lung Cancer Patients With Asbestos Exposure  Anticancer Res  2010  30 (7), 2631-9  PubMed ID: 20682992

8995  Takashi Fukuyama, Yoshinobu Ichiki, Sousuke Yamada, Yoshiki Shigematsu, Tetsuro Baba, Yoshika Nagata, Makiko Mizukami, Masakazu Sugaya, Mitsuhiro Takenoyama, Takeshi Hanagiri, Kenji Sugio, Kosei Yasumoto  Cytokine Production of Lung Cancer Cell Lines: Correlation Between Their Production and the Inflammatory/Immunological Responses Both in Vivo and in Vitro  Cancer Sci  2007  98 (7), 1048-54  PubMed ID: 17511773   DOI: 10.1111/j.1349-7006.2007.00507.x

9004  Makiko Mizukami, Takeshi Hanagiri, Manabu Yasuda, Koji Kuroda, Yoshiki Shigematsu, Tetsuro Baba, Takashi Fukuyama, Yoshika Nagata, Tetsuya So, Yoshinobu Ichiki, Masakazu Sugaya, Tomoko So, Mitsuhiro Takenoyama, Kenji Sugio, Kosei Yasumoto  Antitumor Effect of Antibody Against a SEREX-defined Antigen (UOEH-LC-1) on Lung Cancer Xenotransplanted Into Severe Combined Immunodeficiency Mice  Cancer Res  2007  67 (17), 8351-7  PubMed ID: 17804751   DOI: 10.1158/0008-5472.CAN-06-3889

7413  Mizukami M, Hanagiri T, Baba T, Fukuyama T, Nagata Y, So T, Ichiki Y, Sugaya M, Yasuda M, Takenoyama M, Sugio K, Yasumoto K.  Identification of tumor associated antigens recognized by IgG from tumor-infiltrating B cells of lung cancer: correlation between Ab titer of the patient's sera and the clinical course.  Cancer Sci  2005  96(12):882-8  PubMed ID: 16367908   DOI: 10.1111/j.1349-7006.2005.00119.x

3626  So T, Takenoyama M, Sugaya M, Yasuda M, Eifuku R, Yoshimatsu T, Hanagiri T, Oyama T, Kodate M, Osaki T, Yasumoto K.  Generation of autologous tumor-specific T cell clones from a patient with adenosquamous carcinoma of the lung.  Jpn J Clin Oncol  2001  31(7):311-7  PubMed ID: 11518743   DOI: 10.1093/jjco/hye062

3636  Takenoyama M, Yoshino I, Eifuku R, So T, Imahayashi S, Sugaya M, Yasuda M, Inoue M, Ichiyoshi Y, Osaki T, Nagashima A, Nomoto K, Yasumoto K.  Successful induction of tumor-specific cytotoxic T lymphocytes from patients with non-small cell lung cancer using CD80-transfected autologous tumor cells.  Jpn J Cancer Res  2001  92(3):309-15  PubMed ID: 11267941   DOI: 10.1111/j.1349-7006.2001.tb01096.x

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User's Publication

22524  Kasai F, Fukushima M, Miyagi Y, Nakamura Y.  Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan  Hum Cell  2024  37(4):944-950  PubMed ID: 38639832   DOI: 10.1007/s13577-024-01055-0

11040  Koh Furugaki, Marie Mochizuki, Mirei Kohno, Sei Shu, Naoki Harada, Yasushi Yoshimura   Expression of C-terminal ALK, RET, or ROS1 in lung cancer cells with or without fusion  BMC Cancer  2019  19(1):301  PubMed ID: 30943926   DOI: 10.1186/s12885-019-5527-2