Cell No. : Cell Name
RCB2815 : P20.1
update : 2024/01/17
|
| Comment | Hybridoma producing monoclonal antibody against a peptide, GYPGQV. |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Protein Sci. 2008;17:2120-2126) . 2) RECIPIENT who wants you use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University (Technology Transfer Division) . 3) If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE , the RECIPIENT must contact DEPOSITOR to negotiate. 4) Upon the publishment of the research results obtained through the use of the BIOLOGICAL RESOURCE, the RECIPIENT must send a copy of the published document to DEPOSITOR. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takagi, Junichi
|
| Originator |
Takagi, Junichi
|
| Year of deposit |
2008
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_K262
|
|
hybridoma
|
Fusion_cell_1 name |
SP2/0
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen cell
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
|
Fusion method |
PEG, HAT selection
|
|
Antibody name |
P20.1
|
| Subclass |
IgG1, λ
|
| Immunogen |
residue peptide (NH2-GGDDSTPSILPAPRGYPGQV-cys-CHOOH)
|
|
Antigen_name |
PAR4
|
| Antigen_character |
G-rotein coupled receptors (GPCRs)
|
| Antigen_epitope |
GYPGQV
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
1
|
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