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Cell No. : Cell Name
RCB2815 : P20.1  update : 2021/05/20
CommentHybridoma producing monoclonal antibody against a peptide, GYPGQV.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Protein Sci. 2008;17:2120-2126) . 2) RECIPIENT who wants you use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University (Technology Transfer Division) . 3) If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE , the RECIPIENT must contact DEPOSITOR to negotiate. 4) Upon the publishment of the research results obtained through the use of the BIOLOGICAL RESOURCE, the RECIPIENT must send a copy of the published document to DEPOSITOR.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takagi, Junichi
Originator Takagi, Junichi
Year of deposit 2008
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_K262
hybridoma Fusion_cell_1 name SP2/0
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name P20.1
Subclass IgG1, λ
Immunogen residue peptide (NH2-GGDDSTPSILPAPRGYPGQV-cys-CHOOH)
Antigen_name PAR4
Antigen_character G-rotein coupled receptors (GPCRs)
Antigen_epitope GYPGQV
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3624  Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J.  Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification.  Protein Sci  2008  17(12):2120-6  PubMed ID: 18787202

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User's Publication
9886  Nogi T, Mihara E, Yasui N, Takagi J.  Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System.  Methods Mol. Biol.  2017  1493:57-72  PubMed ID: 27787842



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