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Cell No. : Cell Name
RCB2815 : P20.1  update : 2018/12/05
CommentHybridoma producing monoclonal antibody against a peptide, GYPGQV.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Protein Sci. 2008;17:2120-2126) . 2) RECIPIENT who wants you use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University (Technology Transfer Division) . 3) If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE , the RECIPIENT must contact DEPOSITOR to negotiate. 4) Upon the publishment of the research results obtained through the use of the BIOLOGICAL RESOURCE, the RECIPIENT must send a copy of the published document to DEPOSITOR.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Takagi, Junichi
Originator Takagi, Junichi
Year of deposit 2008
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name SP2/0
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name P20.1
Subclass IgG1, λ
Immunogen residue peptide (NH2-GGDDSTPSILPAPRGYPGQV-cys-CHOOH)
Antigen_name PAR4
Antigen_character G-rotein coupled receptors (GPCRs)
Antigen_epitope GYPGQV
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3624  Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J.  Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification.  Protein Sci  2008  17(12):2120-6  PubMed ID: 18787202

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User's Publication
9886  Nogi T, Mihara E, Yasui N, Takagi J.  Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System.  Methods Mol. Biol.  2017  1493:57-72  PubMed ID: 27787842



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