Cell No. : Cell Name
RCB2813 : NMuMG/Fucci
update : 2024/08/14
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| Comment | A subline of the NMuMG cell line expressing Fucci, a cell cycle marker. Cell line name : NMuMG/Fucci #1.Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | |
| Terms and conditions | 1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE，a citation of the following literature is necessary. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S，Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132:487-498. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyawaki, Atsushi
|
| Originator |
Miyawaki, Atsushi
|
| Year of deposit |
2008
|
| Another name |
NMuMG-Fucci
|
| Original cell |
NMuMG
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NAMRU
|
| Gender |
Female
|
| Tissue |
mammary gland
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| Classification |
transformed
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| Recombinant |
recombinant
|
| Exogene |
Fucci
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_E069
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
2
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