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Cell No. : Cell Name
RCB2813 : NMuMG/Fucci  update : 2021/05/20
CommentA subline of the NMuMG cell line expressing Fucci, a cell cycle marker. Cell line name : NMuMG/Fucci #1.Important notice regarding nomenclature of Fucci.
Comment from the depositor
Terms and conditions1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S,Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132:487-498. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2008
Another name NMuMG-Fucci
Animal mouse < Mammals
Strain name NAMRU
Gender Female
Tissue mammary gland
Classification transformed
Recombinant recombinant
Exogene Fucci
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E069
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
4042  Sakaue-Sawano A1, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A.  Visualizing spatiotemporal dynamics of multicellular cell-cycle progression  Cell  2008  132:487-498  PubMed ID: 18267078

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User's Publication
13313  Sugihara K, Yamaguchi Y, Usui S, Nashimoto Y, Hanada S, Kiyokawa E, Uemura A, Yokokawa R, Nishiyama K, Miura T.  A new perfusion culture method with a self-organized capillary network.  PLoS One  2020    PubMed ID: 33112918
8179  Kawai S, Abiko Y, Amano A.  Odd-skipped related 2 regulates genes related to proliferation and development.  Biochem. Biophys. Res. Commun.  2010  398:184-90  PubMed ID: 20599744



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