Cell No. : Cell Name RCB2813 : NMuMG/Fucci  update : 2025/01/15
Comment			A subline of the NMuMG cell line expressing Fucci, a cell cycle marker. Cell line name : NMuMG/Fucci #1.Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositor
Terms and conditions			1)The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., Cell 132 (3): 487-498 (2008).
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Miyawaki, Atsushi
		Originator	Miyawaki, Atsushi
		Year of deposit	2008
		Another name	NMuMG-Fucci
		Original cell	NMuMG
		Animal	_mouse < Mammals
		Strain name	NAMRU
		Gender	Female
		Tissue	mammary gland
		Classification	transformed
		Recombinant	recombinant
		Exogene	Fucci
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_E069
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (high glucose) + 10% FBS + 10μg/ml Insulin
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

4042  Sakaue-Sawano A1, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A.  Visualizing spatiotemporal dynamics of multicellular cell-cycle progression  Cell  2008  132:487-498  PubMed ID: 18267078   DOI: 10.1016/j.cell.2007.12.033

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User's Publication

13313  Sugihara K, Yamaguchi Y, Usui S, Nashimoto Y, Hanada S, Kiyokawa E, Uemura A, Yokokawa R, Nishiyama K, Miura T.  A new perfusion culture method with a self-organized capillary network.  PLoS One  2020    PubMed ID: 33112918   DOI: 10.1371/journal.pone.0240552

8179  Kawai S, Abiko Y, Amano A.  Odd-skipped related 2 regulates genes related to proliferation and development.  Biochem. Biophys. Res. Commun.  2010  398:184-90  PubMed ID: 20599744   DOI: 10.1016/j.bbrc.2010.06.054