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Cell No. : Cell Name
RCB2812 : HeLa/Fucci  update : 2022/03/23
CommentA subline of the HeLa cell line expressing Fucci, a cell cycle marker. Cell line name : HeLa/Fucci #4.Important notice regarding nomenclature of Fucci.
Comment from the depositor
Terms and conditions1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S,Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132:487-498. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2008
Another name HeLa.S-Fucci
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E070
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 40


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Reference
4042  Sakaue-Sawano A1, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A.  Visualizing spatiotemporal dynamics of multicellular cell-cycle progression  Cell  2008  132:487-498  PubMed ID: 18267078
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

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User's Publication
20695  Nagy ÁG, Kanyó N, Vörös A, Székács I, Bonyár A, Horvath R.  Population distributions of single-cell adhesion parameters during the cell cycle from high-throughput robotic fluidic force microscopy.  Sci Rep  2022  12(1):7747  PubMed ID: 35546603
20738  Yamamoto M, Miyata S.  Influences of Microscopic Imaging Conditions on Accuracy of Cell Morphology Discrimination Using Convolutional Neural Network of Deep Learning.  Micromachines (Basel)  2022  13(5)  PubMed ID: 35630227
20336  Ungai-Salánki R, Haty E, Gerecsei T, Francz B, Béres B, Sztilkovics M, Székács I, Szabó B, Horvath R.  Single-cell adhesion strength and contact density drops in the M phase of cancer cells.  Sci Rep  2021  11(1):18500  PubMed ID: 34531409
16053  Fukunaga H, Kaminaga K, Hirose E, Watanabe R, Usami N, Prise KM, Yokoya A.  No Intercellular Regulation of the Cell Cycle among Human Cervical Carcinoma HeLa Cells Expressing Fluorescent Ubiquitination-Based Cell-Cycle Indicators in Modulated Radiation Fields  Int J Mol Sci  2021  ;22(23):12785  PubMed ID: 34884589
13522  Herr P, Boström J, Rullman E, Rudd SG, Vesterlund M, Lehtiö J, Helleday T, Maddalo G, Altun M.  Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics  Mol Cell Proteomics  2020   19(4):608-623  PubMed ID: 32051232
6925  Nojima H, Homma H, Onozato Y, Kaida A, Harada H, Miura M.  Differential properties of mitosis-associated events following CHK1 and WEE1 inhibitor treatments in human tongue carcinoma cells.  Exp Cell Res  2020  386(2):111720  PubMed ID: 31738907
6916  Hitora Y, Takada K, Ise Y, Woo SP, Inoue S, Mori N, Takikawa H, Nakamukai S, Okada S, Matsunaga S.  Metachromins X and Y from a marine sponge Spongia sp. and their effects on cell cycle progression.  Bioorg Med Chem  2020  28(2):115233  PubMed ID: 31848114
11649  Goto T, Homma H, Kaida A, Miura M.  WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells.  J. Radiat. Res.  2019    PubMed ID: 31347653
11840  Homma H, Nojima H, Kaida A, Miura M.  Induction of endomitosis-like event in HeLa cells following CHK1 inhibitor treatment.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31610912
10709  Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler DJ, Badri S, LaCava J, Ueberheide B, Yun CY, Fenyö D, Boeke JD.  LINE-1 protein localization and functional dynamics during the cell cycle.  Elife  2018  7:  PubMed ID: 29309036
11174  Manila NG, Kaida A, Nakahama KI, Miura M.  Insulin-like growth factor I receptor regulates the radiation-induced G2/M checkpoint in HeLa cells.  Biochem. Biophys. Res. Commun.  2018  503(4):2977-2983  PubMed ID: 30119887
11230  Li SC, Stucky A, Chen X, Kabeer MH, Loudon WG, Plant AS, Torno L, Nangia CS, Cai J, Zhang G, Zhong JF.  Single-cell transcriptomes reveal the mechanism for a breast cancer prognostic gene panel.  Oncotarget  2018    PubMed ID: 30279960
11348  D'Alessandro G, Whelan DR, Howard SM, Vitelli V, Renaudin X, Adamowicz M, Iannelli F, Jones-Weinert CW, Lee M, Matti V, Lee WTC, Morten MJ, Venkitaraman AR, Cejka P, Rothenberg E, d'Adda di Fagagna F.  BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment.  Nat Commun  2018    PubMed ID: 30560944
9857  Izumi Y, Fujii K, Yamamoto S, Matsuo K, Namatame H, Taniguchi M, Yokoya A.  DNA damage response induces structural alterations in histone H3-H4.  J. Radiat. Res.  2017  58:59-65  PubMed ID: 27672100
10101  Kobayashi D, Kakinouchi K, Nagae T, Nagai T, Shimura K, Hazama A.  Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism.  FEBS Lett.  2017  591:718-727  PubMed ID: 28134446
10655  Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M.  Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.  PLoS ONE  2017  12:e0188772  PubMed ID: 29228002
10978    Effect of metal–metal distance in Ni(II)-based metallo-supramolecular polymers: DNA binding and cytotoxicity  RSC Adv  2017  7, 38008-38013 
14778  Paolo Mita, Aleksandra Wudzinska, Xiaoji Sun, Joshua Andrade, Shruti Nayak, David J. Kahler, Sana Badri, John LaCava, Beatrix Ueberheide, Chi Y. Yun, David Fenyo, Jef D. Boeke  LINE-1 and the cell cycle: protein localization and functional dynamics  bioRxiv  2017  June 30, 2017 
14780  Akinobu Honda, Petros K. Chigwechokha, Rena Wakamatsu, Yuko Kamada, Masaharu Komastu, Kazuhiro Shiozaki  Characterization of tilapia sialidase and its significance in bacterial infection  The JSFS 85th Anniversary-Commemorative International Symposium  2017  09007 
16628  Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Tachibana M, Mizuguchi H.  MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2  J Virol  2017  91(12):e00159-17  PubMed ID: 28356525
9787  Adamowicz M, Vermezovic J, d'Adda di Fagagna F.  NOTCH1 Inhibits Activation of ATM by Impairing the Formation of an ATM-FOXO3a-KAT5/Tip60 Complex.  Cell Rep  2016  16:2068-2076  PubMed ID: 27524627
10130  Eka Sunarwidhi Prasedya, Masao Miyake, Daisuke Kobayashi, Akihiro Hazama  Carrageenan Delays Cell Cycle Progression in Human Cancer Cells in Vitro Demonstrated by FUCCI Imaging  BMC Complement Altern Med  2016  16:270  PubMed ID: 27487950
12667  Kaminaga K, Noguchi M, Narita A, Hattori Y, Usami N, Yokoya A.  Cell cycle tracking for irradiated and unirradiated bystander cells in a single colony with exposure to a soft X-ray microbeam  Int J Radiat Biol  2016  92(11):739-744  PubMed ID: 27537347
13981  Izumi Y, Fujii K, Wien F, Houée-Lévin C, Lacombe S, Salado-Leza D, Porcel E, Masoud R, Yamamoto S, Réfrégiers M, Hervé du Penhoat MA, Yokoya A.  Structure Change from β-Strand and Turn to α-Helix in Histone H2A-H2B Induced by DNA Damage Response  Biophys J  2016  111(1):69-78  PubMed ID: 27410735
14190  C. Barent, L. Niederreiter, L. Pack. Heselich, B. Jakob, G. Taucher-Scholz, and N. B. Averbeck  RNF138 stimulates DNA-end resection upon heavy-ion-irradiation in human G1-phase cells  GSI SCIENTIFIC REPORT 2016  2016  308 
7179  Izumi Y, Yamamoto S, Fujii K, Yokoya A.  Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours.  Radiat. Res.  2015  184:554-8  PubMed ID: 26488755
7394  Kaminaga K, Noguchi M, Narita A, Sakamoto Y, Kanari Y, Yokoya A.  Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators.  Radiat Prot Dosimetry  2015  166:91-4  PubMed ID: 25877544
12547  Okuyama K, Kaida A, Hayashi Y, Hayashi Y, Harada K, Miura M.  KPU-300, a Novel Benzophenone-Diketopiperazine-Type Anti-Microtubule Agent with a 2-Pyridyl Structure, Is a Potent Radiosensitizer That Synchronizes the Cell Cycle in Early M Phase  PLoS One  2015  10(12):e0145995  PubMed ID: 26716455
15449  Inoue M, Yoshimura M, Kobayashi M, Morinibu A, Itasaka S, Hiraoka M, Harada H.  PLK1 blockade enhances therapeutic effects of radiation by inducing cell cycle arrest at the mitotic phase  Sci Rep  2015  5:15666  PubMed ID: 26503893
12985  Tsuchida E, Kaida A, Pratama E, Ikeda MA, Suzuki K, Harada K, Miura M.  Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell-Based Kinetics in an Asynchronous Cell Population  PLoS One  2015  10(6):e0128090  PubMed ID: 26086724
9045  Kaida A, Miura M.  Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells  Cancer Sci   2015  106(10):1370-6  PubMed ID: 26195156
8411  Goto T, Kaida A, Miura M.  Visualizing cell-cycle kinetics after hypoxia/reoxygenation in HeLa cells expressing fluorescent ubiquitination-based cell cycle indicator (Fucci)  Exp Cell Res  2015  339(2):389-96  PubMed ID: 26500111
8114  B Gao, T Konno, K Ishihara  Phospholipid Polymer Multilayered Hydrogels Containing Cells for Cancer Drug Screening  Transactions of the Materials Research Society of Japan  2015  40:59-63 
4080  Yamada C, Kaida A, Okuyama K, Harada K, Miura M  Effects of Chk1 inhibitor or paclitaxel on cisplatin-induced cell-cycle kinetics and survival in parental and cisplatin-resistant HeLa cells expressing fluorescent ubiquitination-based cell cycle indicator (Fucci).  Integrative Cancer Science and Therapeutics  2015  2:87-92 
15940  Nagano T, Hashimoto T, Nakashima A, Hisanaga S, Kikkawa U, Kamada S.  Cyclin I is involved in the regulation of cell cycle progression  Cell Cycle  2013  12(16):2617-24  PubMed ID: 23907122
15942  Tatsuo Aikawa, Tomohiro Konno, Kazuhiko Ishihara  Phospholipid polymer hydrogel microsphere modulates the cell cycle profile of encapsulated cells  Soft Matter  2013  9:4628 
7637  Kaida A, Miura M.  Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci).  Exp. Cell Res.  2012  318:288-97  PubMed ID: 22079518
13673  Ridenour DA, McKinney MC, Bailey CM, Kulesa PM.  CycleTrak: a novel system for the semi-automated analysis of cell cycle dynamics  Dev Biol  2012  365(1):189-95  PubMed ID: 22387844
15973  Kaida A, Miura M.  Differential dependence on oxygen tension during the maturation process between monomeric Kusabira Orange 2 and monomeric Azami Green expressed in HeLa cells  Biochem Biophys Res Commun  2012  421(4):855-9  PubMed ID: 22554525
7770  Nakayama M, Kaida A, Deguchi S, Sakaguchi K, Miura M.  Radiosensitivity of early and late M-phase HeLa cells isolated by a combination of fluorescent ubiquitination-based cell cycle indicator (Fucci) and mitotic shake-off.  Radiat. Res.  2011  176:407-11  PubMed ID: 21692656



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