Cell No. : Cell Name
RCB2812 : HeLa/Fucci
update : 2019/02/27
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| Comment | A subline of the HeLa cell line expressing Fucci, a cell cycle marker. Cell line name : HeLa/Fucci #4.Important notice regarding nomenclature of Fucci. |
| Comment from the depositor | |
| Terms and conditions | 1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE，a citation of the following literature is necessary. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S，Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132:487-498. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Miyawaki, Atsushi
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| Originator |
Miyawaki, Atsushi
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| Year of deposit |
2008
|
| Another name |
HeLa.S-Fucci
|
| Animal |
human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Female
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| Tissue |
cervix
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| Case history |
cervical carcinoma
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| Classification |
cancer
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| Recombinant |
recombinant
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| Exogene |
Fucci
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| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
19
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| User's Publication |
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6925
Nojima H, Homma H, Onozato Y, Kaida A, Harada H, Miura M.
Differential properties of mitosis-associated events following CHK1 and WEE1 inhibitor treatments in human tongue carcinoma cells.
Exp Cell Res
2020
386(2):111720
PubMed ID: 31738907
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6916
Hitora Y, Takada K, Ise Y, Woo SP, Inoue S, Mori N, Takikawa H, Nakamukai S, Okada S, Matsunaga S.
Metachromins X and Y from a marine sponge Spongia sp. and their effects on cell cycle progression.
Bioorg Med Chem
2020
28(2):115233
PubMed ID: 31848114
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11649
Goto T, Homma H, Kaida A, Miura M.
WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells.
J. Radiat. Res.
2019
PubMed ID: 31347653
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11840
Homma H, Nojima H, Kaida A, Miura M.
Induction of endomitosis-like event in HeLa cells following CHK1 inhibitor treatment.
Biochem. Biophys. Res. Commun.
2019
PubMed ID: 31610912
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10709
Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler DJ, Badri S, LaCava J, Ueberheide B, Yun CY, Fenyö D, Boeke JD.
LINE-1 protein localization and functional dynamics during the cell cycle.
Elife
2018
7:
PubMed ID: 29309036
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11174
Manila NG, Kaida A, Nakahama KI, Miura M.
Insulin-like growth factor I receptor regulates the radiation-induced G2/M checkpoint in HeLa cells.
Biochem. Biophys. Res. Commun.
2018
503(4):2977-2983
PubMed ID: 30119887
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11230
Li SC, Stucky A, Chen X, Kabeer MH, Loudon WG, Plant AS, Torno L, Nangia CS, Cai J, Zhang G, Zhong JF.
Single-cell transcriptomes reveal the mechanism for a breast cancer prognostic gene panel.
Oncotarget
2018
PubMed ID: 30279960
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11348
D'Alessandro G, Whelan DR, Howard SM, Vitelli V, Renaudin X, Adamowicz M, Iannelli F, Jones-Weinert CW, Lee M, Matti V, Lee WTC, Morten MJ, Venkitaraman AR, Cejka P, Rothenberg E, d'Adda di Fagagna F.
BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment.
Nat Commun
2018
PubMed ID: 30560944
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4090
B. Cameron, G. Traver, M.L. Freeman, J. Wang, K. Sekhar, L. Johnson, D. Dean, M. Fahhoum
Intrinsic Resistance of V-SVZ Neural Stem Cells Subjected to Concurrent Daily Radiation and Temozolomide Therapy
International Journal of Radiation Oncology
2018
102:e145
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9857
Izumi Y, Fujii K, Yamamoto S, Matsuo K, Namatame H, Taniguchi M, Yokoya A.
DNA damage response induces structural alterations in histone H3-H4.
J. Radiat. Res.
2017
58:59-65
PubMed ID: 27672100
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10101
Kobayashi D, Kakinouchi K, Nagae T, Nagai T, Shimura K, Hazama A.
Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism.
FEBS Lett.
2017
591:718-727
PubMed ID: 28134446
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10655
Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M.
Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.
PLoS ONE
2017
12:e0188772
PubMed ID: 29228002
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10978
Effect of metal–metal distance in Ni(II)-based metallo-supramolecular polymers: DNA binding and cytotoxicity
RSC Adv
2017
7, 38008-38013
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9787
Adamowicz M, Vermezovic J, d'Adda di Fagagna F.
NOTCH1 Inhibits Activation of ATM by Impairing the Formation of an ATM-FOXO3a-KAT5/Tip60 Complex.
Cell Rep
2016
16:2068-2076
PubMed ID: 27524627
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10130
Eka Sunarwidhi Prasedya, Masao Miyake, Daisuke Kobayashi, Akihiro Hazama
Carrageenan Delays Cell Cycle Progression in Human Cancer Cells in Vitro Demonstrated by FUCCI Imaging
BMC Complement Altern Med
2016
16:270
PubMed ID: 27487950
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7179
Izumi Y, Yamamoto S, Fujii K, Yokoya A.
Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours.
Radiat. Res.
2015
184:554-8
PubMed ID: 26488755
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7394
Kaminaga K, Noguchi M, Narita A, Sakamoto Y, Kanari Y, Yokoya A.
Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators.
Radiat Prot Dosimetry
2015
166:91-4
PubMed ID: 25877544
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7637
Kaida A, Miura M.
Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci).
Exp. Cell Res.
2011
318:288-97
PubMed ID: 22079518
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7770
Nakayama M, Kaida A, Deguchi S, Sakaguchi K, Miura M.
Radiosensitivity of early and late M-phase HeLa cells isolated by a combination of fluorescent ubiquitination-based cell cycle indicator (Fucci) and mitotic shake-off.
Radiat. Res.
2011
176:407-11
PubMed ID: 21692656
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