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Cell No. : Cell Name
RCB2811 : 7F9 (hybridoma)  update : 2021/05/20
CommentHybridoma. Anti-Rat Carboxypeptidase M MoAb producing.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Respirology 2007;12:54-62) .
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Basic information Depositor Fijiwara, Naoyuki
Originator Fijiwara, Naoyuki
Year of deposit 2008
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U638
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name PAI
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name 7F9
Subclass IgG
Immunogen embryonal day 18.5 rat lungs
Antigen_name carboxypeptidase-M
Antigen_character embryonal day 18.5 rat lungs
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10mM HEPES + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 55μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3623  Fujiwara N, Ikeda M, Hirabayashi S, Mori H, Shirasawa M, Kansaku A, Sunamori M, Hata Y.  Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase-M in developing and adult rat lung.  Respirology  2007  12(1):54-62  PubMed ID: 17207026

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