Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB2811 : 7F9 (hybridoma)  update : 2021/05/20
CommentHybridoma. Anti-Rat Carboxypeptidase M MoAb producing.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Respirology 2007;12:54-62) .
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fijiwara, Naoyuki
Originator Fijiwara, Naoyuki
Year of deposit 2008
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U638
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name PAI
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name 7F9
Subclass IgG
Immunogen embryonal day 18.5 rat lungs
Antigen_name carboxypeptidase-M
Antigen_character embryonal day 18.5 rat lungs
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10mM HEPES + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 55μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


To topTop
Reference
3623  Fujiwara N, Ikeda M, Hirabayashi S, Mori H, Shirasawa M, Kansaku A, Sunamori M, Hata Y.  Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase-M in developing and adult rat lung.  Respirology  2007  12(1):54-62  PubMed ID: 17207026

To topTop
User's Publication



Back Back Return Top Page