Cell No. : Cell Name
RCB2811 : 7F9 (hybridoma)
update : 2024/01/17
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| Comment | Hybridoma. Anti-Rat Carboxypeptidase M MoAb producing. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Respirology 2007;12:54-62) . |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fijiwara, Naoyuki
|
| Originator |
Fijiwara, Naoyuki
|
| Year of deposit |
2008
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_U638
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cell
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
PAI
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
|
Fusion method |
PEG, HAT selection
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|
Antibody name |
7F9
|
| Subclass |
IgG
|
| Immunogen |
embryonal day 18.5 rat lungs
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|
Antigen_name |
carboxypeptidase-M
|
| Antigen_character |
embryonal day 18.5 rat lungs
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10mM HEPES + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 55μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Antibody subclass |
|
IgG2b
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
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